The possibility of replacing the rabbit pyrogen test by the Limulus Amebocyte lysate (LAL) test, as a final release test for Large Volume Parenterals (LVPs) was investigated. The sensitivity and specificity of the LAL test and rabbit pyrogen assay were studied by means of artificially contaminated parenterals. Various LVPs (mostly containing electrolytes) were spiked with 0.5 and 5 EUlml o...

The finding of 3,4-dihydroxyphenylacetic acid (DHPAA) in the urine of rabbits after the oral ingestion of rutin or its aglycone quercetin has been reported from this Laboratory (1). In this same report it was stated that in each case after the administration of the flavonoid an examination of paper chromatograms of the ether extracts of the urine revealed the excretion of at least four metaboli...

BACKGROUND The parasite Schistosoma japonicum causes schistosomiasis disease, which threatens human life and hampers economic and social development in some Asian countries. An important lesson learned from efforts to reduce the occurrence of schistosomiasis is that the diagnostic approach must be altered as further progress is made towards the control and ultimate elimination of the disease. ...

The finding of 3,4-dihydroxyphenylacetic acid (DHPAA) in the urine of rabbits after the oral ingestion of rutin or its aglycone quercetin has been reported from this Laboratory (1). In this same report it was stated that in each case after the administration of the flavonoid an examination of paper chromatograms of the ether extracts of the urine revealed the excretion of at least four metaboli...

Background and Aims: The protozoan parasites of the genus Leishmania are the causative agents of various clinical diseases. Different methods of cultivation of Leishmania parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of Leishmania major. Materials and Met...

Ab initio simulations of a range interferometric experiments are used to identify strong dependence on multiphoton phase shifts in above-threshold ionization. A simple rule thumb for interaction is derived explain both the conservation photoelectron yield and its absolute CEP-dependence. For instance, it found that ionization shifted by $\pi/4$ relative RABBIT experiments, there no RABBIT-term ...

Rabbit haemorrhagic disease virus emerged in China in 1984, and has killed hundreds of millions of wild rabbits in Australia and Europe. In the UK there appears to be an endemic non-pathogenic strain, with high levels of seroprevalence being recorded, in the absence of associated mortality. Using a seasonal, age-structured model we examine the hypothesis that differences in rabbit population de...

Many researches have been conducted in experimental models in order to study the development of atherosclerosis from hyperlipidemia-inducing diets. Since rabbits are very sensitive to cholesterol-rich diets and accumulate large amounts of cholesterol in their plasma, their use as experimental models to evaluate the development of atherosclerosis is highly relevant and brings information on fact...

Macrophages can change their phenotype in response to environmental cues. Polarized macrophages are broadly classified into two groups: classical activated M1 and alternative activated M2. Characterization of human macrophages has been widely studied, but polarized macrophages in rabbits have not been characterized. We characterized rabbit macrophages that were polarized using human recombinant...

A comparative study of rabbit pyrogen test and human whole blood assay was performed on released preparations of human serum albumin. In addition, the samples were spiked with 5 IU/ml (in whole blood 0.5 IU/ml too) and 10 IU endotoxin/ml. The unspiked samples were negative in both assays. The human whole blood test resulted in the same level of security for the products as the rabbit pyrogen te...