Visualization of chromosomal dynamics is important for understanding many fundamental intra-nuclear processes. Efficient and reliable live-cell multicolor labeling of chromosomal loci can realize this goal. However, the current methods are constrained mainly by insufficient labeling throughput, efficiency, flexibility as well as photostability. Here we have developed a new approach to realize d...

As the size of data growing at a rapid pace, clustering a very large data set inevitably incurs a time-consuming process. To improve the efficiency of clustering, sampling is usually used to scale down the size of data set. However, with sampling applied, how to allocate unlabeled objects into proper clusters is a very difficult problem. In this paper, based on the frequency of attribute values...

The establishment of a functional blood vessel network is an essential part of organogenesis, and is required for optimal organ function. For example, in the thymus proper vasculature formation and patterning is essential for thymocyte entry into the organ and mature T-cell exit to the periphery. The spatial arrangement of blood vessels in the thymus is dependent upon signals from the local mic...

A new model-based automatic prosody labeling method for Mandarin speech is proposed. It first introduces four models to describe the relationships of the prosody tags to be labeled, the prosodic features of the speech signals, and the linguistic features of the associated texts. It then employs a sequential optimization procedure to estimate parameters of these four models and find all prosody ...

‎a $(p‎,‎q)$ graph $g$ is said to have a $k$-odd mean‎ ‎labeling $(k ge 1)$ if there exists an injection $f‎ : ‎v‎ ‎to {0‎, ‎1‎, ‎2‎, ‎ldots‎, ‎2k‎ + ‎2q‎ - ‎3}$ such that the‎ ‎induced map $f^*$ defined on $e$ by $f^*(uv) =‎ ‎leftlceil frac{f(u)+f(v)}{2}rightrceil$ is a‎ ‎bijection from $e$ to ${2k - ‎‎‎1‎, ‎2k‎ + ‎1‎, ‎2k‎ + ‎3‎, ‎ldots‎, ‎2‎ ‎k‎ + ‎2q‎ - ‎3}$‎. ‎a graph that admits $k$...

The specificity of labeling enteroviruses with (32)P-labeled NaH(2)PO(4) or (14)C-leucine can be determined by comparing the percent adsorption of infective particles and radioactivity to membrane (Millipore) filters.

Identification of protein-protein interactions is a major goal of biological research. Despite technical advances over the last two decades, important but still largely unsolved challenges include the high-throughput detection of interactions directly from primary tissue and the identification of interactors of insoluble proteins that form higher-order structures. We have developed a novel, pro...

The terminal deoxynucleotide transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) method for monitoring targeted cell ablation is based on the in situ labeling of DNA fragmentation sites in nuclei of intact fixed cells. Unlike other methods of detecting dying cells, the use of fixed material allows antigen expression to be monitored at the same time that apoptosis is confirmed in the targe...

Arterial spin labeling is a versatile perfusion quantification methodology, which has the potential to provide accurate characterization of cerebral blood flow (CBF) in mouse models. However, a paucity of physiological data needed for accurate modeling, more stringent requirements for gradient performance, and strong artifacts introduced by magnetization transfer present special challenges for ...