Medicago sativa (alfalfa) is a widely used animal feed. However, its quality has been difficult to control due the lack of appropriate marker compounds. Therefore, it very necessary select an (Q-marker) quality. In this study, medium-pressure liquid chromatography and high-pressure were employed effectively prepare separation Q-marker (L-tryptophan) from sativa. Firstly, using MCI GEL® CHP20P a...

An investigation was carried out on the effect of some amino acids on the growth and production of Lglutamic acid by a mutant Micrococcus glutamicus AB100 using selected suitable synthetic medium. It was observed that among different amino acids studied, l-alanine, l-arginine, l-threonine, l-tryptophan, l-proline and l-hitidine had positive effect; l-leucine, l-valine, l-phenylalanine, l-serine...

1. The effects of the administration of oestradiol and vitamin B, on tryptophan metabolism in the rat have been assessed by measurement of the release of 14C0, from [14C]tryptophan, in vivo, in order to determine whether, and to what extent, the abnormalities of tryptophan metabolism that are associated with oestrogen administration can be attributed to drug-induced vitamin B, deficiency or dep...

The influence of a tryptophan enriched diet (L-tryptophan added as 1% of total diet), fed over 10 days, on the rat duodenum and pancreas was studied by immunohistology, measurements of serotonin and tryptophan tissue concentrations by HPLC, and incubations of pancreatic lobules. Ingestion of a tryptophan enriched diet resulted in increased contents of tryptophan and serotonin in the duodenum th...

In animals, liver tryptophan pyrrolase was induced independently by administration of its substrate L-tryptophan (I), and of the hormone hydrocortisone (2). Some analogues of tryptophan which are not substrates of the tryptophan pyrrolase, such as c+mcthyl-DL-tryptophan (3) and D-tryptophan,’ have been reported to induce the enzyme in intact animals. Since a substance may cause enzyme induction...

The inability of vitamin Be-deficient rats and mice to convert dietary tryptophan to nicotinic acid and its metabolites has been observed by several workers (l-4). Further, when vitamin Be supplements were given to rats previously deficient in the vitamin, the animals usually recovered the ability to transform tryptophan into nicotinic acid metabolites, although the response was apparently infl...

The enhanced availability of tryptophan in the brain, as a consequence of exogenous tryptophan administration, can increase neuronal serotonin synthesis and this can interfere with brain function. REM sleep deprivation (D) constitutes another external factor that can change brain excitability, facilitating, in some cases, the manifestation of neurological diseases like epilepsy. Here we used co...

BACKGROUND AND OBJECTIVES The essential amino acid L-tryptophan can be produced by a condensation reaction between indole and L-serine, catalyzed by B. subtilis with tryptophan synthase activity. Application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier. MATERIALS AND METHODS The optimum concentration of the Iranian can...

Brain serotonin cocentrations at 1 p.m. were significantly elevated 1 hour after rats received a dose of L-tryptophan (12.5 milligrams per kilogram. intraperitoneally) smaller than one-twentieth of the normal daily dietary intake. Plasma and brain tryptophan levels were elevated 10 to 60 minutes after the injection, but they never exceeded the concentrationis that occur nocturnally in untreated...