Protein export from the nucleus is often mediated by a Leucine-rich Nuclear Export Signal (NES). NESbase is a database of experimentally validated Leucine-rich NESs curated from literature. These signals are not annotated in databases such as SWISS-PROT, PIR or PROSITE. Each NESbase entry contains information of whether NES was shown to be necessary and/or sufficient for export, and whether the...

Rat aortic endothelium is differentiated regionally for three signal pathways capable of regulating the cGMP content of the underlying smooth muscle. Formation of nitric oxide (NO) from L-arginine and of glutamate from L-leucine increase cGMP; however, formation of prostaglandin H2 (PGH2) decreases cGMP. All three have peak activity in the windkessel area just distal to the aortic arch and decr...

A mutant of Escherichia coli K-12 was examined which has growth medium-dependent lysyl-transfer ribonucleic acid (tRNA) ligase activity. In minimal medium or 0.5% yeast extract, the activity of the enzyme in the mutant strain was 5 to 10% of wild type. However, when the mutant was grown in a highly enriched medium, such as AC broth (Difco), the activity of the mutant ligase increased 10- to 20-...

Vancomycin is a glycopeptide antibiotic used for the treatment of serious infections by Gram-positive pathogens. Vancomycin inhibits cell wall biosynthesis by targeting the d-Ala-d-Ala terminus of peptidoglycan (PG). The highly cross-linked heptapeptide aglycon structure of vancomycin is the d-Ala-d-Ala binding site. The first residue of vancomycin is N-methyl-leucine, which is crucial for the ...

GOLDBERG, ALFRED L., AND RICHARD ODESSEY. Oxidation of amino acids by diaphragms from fed and fasted rats. Am. J. Physiol. 223(6) : 1384-1391. 1972.-The oxidation of L-amino-14C acids was studied in isolated diaphragms from fed and fasted rats. During a 90-min incubation, diaphragms from fed rats produced 14C02 from leucinel14C, isoleucine1 -14C, valine1 -14C, alanineP4C, glutamate-U-14C, and a...

The hydrophobic dl-amino acids alanine, valine, leucine, and isoleucine have been cocrystallized with LiCl via solid-state solution methods, the effect of preparation conditions solvent choice on racemic versus conglomerate formation has investigated. For sake comparison, enantiopure l-amino also reacted in same experimental conditions. With dl-alanine only, a ionic cocrystal formula dl-alanine...

Abstract Background Biosynthesis of l - tert -leucine ( -tle), a significant pharmaceutical intermediate, by cofactor regeneration system friendly and efficiently is worthful goal all the time. The leucine dehydrogenase (LeuDH) glucose (GDH) has showed great coupling catalytic efficiency in synthesis -tle, however multi-enzyme complex GDH LeuDH never been constructed successfully. Results In th...

The gene cluster from Amycolotopsis orientalis responsible for biosynthesis of the vancomycin-type glycopeptide antibiotic chloroeremomycin was recently sequenced, indicating that this antibiotic derives from a seven-residue peptide synthesized by a three-subunit (CepA, CepB, and CepC) modular nonribosomal peptide synthetase. Expression of all or parts of the peptide synthetase in Escherichia c...