background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

gliomas represent highly aggressive brain tumors and include glioblastoma as the most common primary brain tumor in adults. This disease is related to a life expectancy of less than 2 years after diagnosis despite best standard of care involving surgery, chemo-and radiotherapy. With a relatively modest, but significant effect on patient survival, the alkylating agent temozolomide is commonly ad...

Asparaginase is an effective treatment for patients with acute lymphocytic leukemia (ALL). Unfortunately, asparaginase therapy is associated with a high incidence of hypersensitivity reactions (up to 73%), including life-threatening anaphylaxis, and its half-life of approximately 20 hours necessitates daily administration. Pegaspargase, a modification of L-asparaginase, has a longer half-life (...

Normal rat liver hepatocytes and the hepatocytes of hepatic nodules induced by N-2-fluorenylacetamide respond to 70% hepatectomy with vigorous mitotic activity. The administration of L-asparaginase at the time of operation delays the mitotic cycle of every normal hepatocyte by 10 to 12 hr. When L-asparaginase was administered to rats which had received N-2-fluorenylacetamide, an 80% inhibition ...

Endophytic microorganisms are recognized as potential source of novel chemical molecules that might be useful in the treatment of infectious diseases. This study was conducted with the aim to isolate and identify endophytic bacteria from medicinal plants recommended for cancer therapy with a potential to produce L-asparaginase. Five different plants were selected to enhance diversity as Cathara...

Of 123 species of bacteria surveyed for L-asparaginase synthesis, Erwinia aroideae NRRL B-138 provided the highest yields.

REFERENCES 1. Clavell LA, Gelber RD, Cohen HJ, et al. Four-agent induction and intensive asparaginase therapy for treatment of childhood acute lymphoblastic leukemia. N Engl J Med 1986;315:657-63. 2. Roesmann A, Afify M, Panse J, Eisert A, Steitz J, Tolba RH. L-carnitine ameliorates L-asparaginase-induced acute liver toxicity in steatotic rat livers. Chemotherapy 2013;59:167-75. 3. Alshiekh-Nas...