نتایج جستجو برای: krebs
تعداد نتایج: 3765 فیلتر نتایج به سال:
BACKGROUND The role of anaplerotic nutrient entry into the Krebs cycle via pyruvate carboxylase has been the subject of increased scrutiny and in particular whether this is dysregulated in cancer. Here, we use a tracer-based NMR analysis involving high-resolution (1)H-(13)C-HSQC spectra to assess site-specific label incorporation into a range of metabolite pools, including malate, aspartate and...
Culture media for mouse preimplantation development are usually derived from two basic solutions: Krebs-Ringer and Tyrode. We have used outbred mice (OF1) to make a comparative analysis of derived media of both types and their success in sustaining development from the one-cell to the blastocyst stage. The best results (up to 50%), in terms of overcoming the two-cell block and sustaining develo...
Hawk, P. B., Oser, B. L. & Summerson, W. H. (1947). Practical Phy8iotogical Chemi8try. London: Churchill. Heilbrunn, L. V. (1947). An Outline of General Phy8iology. Philadelphia: Saunders. Hoagland, H. & Stone, D. (1948). Amer. J. Phy8iol. 152, 423. Klein, J. R. & Olsen, N. S. (1947). J. biol. Chem. 167, 747. Krebs, H. A. (1950). Biochim. biophy8. Acta, Am8t., 4, 249. Krebs, H. A. & Eggleston, ...
13C NMR of isotopically enriched metabolites has been used to study the metabolism of Microbacterium ammoniaphilum, a bacterium which excretes large quantities of L-glutamic acid into the medium. Biosynthesis from 90% [1-13C]glucose results in relatively high specificity of the label, with [2,4-13C2]glutamate as the major product. The predominant biosynthetic pathway for synthesis of glutamate ...
We describe the strategy, which allows curing experimental mice engrafted with Krebs-2 ascites. The strategy is based on the facts that i) Krebs-2 tumor-initiating stem cells (TISCs) are naturally capable of internalizing fragments of extracellular double-stranded DNA (dsDNA); ii) upon delivery into TISCs, these dsDNA fragments interfere with the on-going DNA repair process so that TISCs either...
We have modified the apparatus for isolated rat liver perfusion (IPRL) in order to be able to perform two perfusions simultaneously. In addition, we studied the quality and stability of livers by comparison of five different perfusates: Blood (Group A), Original Krebs Henseleit buffer (Group B), Krebs buffer with glucose (Group C) or bovine serum albumin (BSA) added, (Group D). In a last group ...
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