Genetic variation within nuclear and mitochondrial DNA of Meloidogyne species and host races has been evaluated for the development of root-knot nematode molecular diagnostics. This review summarizes the distinctive features of several useful DNA-based assays for plant-parasitic nematodes, focusing upon the direct application of these procedures for Meloidogyne detection, identification, and sy...

Pre-plant soil fumigation with methyl bromide and host resistance were compared for managing the southern root-knot nematode (Meloidogyne incognita) in pepper. Three pepper cultivars (Carolina Cayenne, Keystone Resistant Giant, and California Wonder) that differed in resistance to M. incognita were grown in field plots that had been fumigated with methyl bromide (98% CHBr : 2% CClNO [w/w]) befo...

Background: The root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood reniform Rotylenchulus reniformis (Linford Oliveiro) have been found consistently associated with plant damage reduced yield of chickpea (Cicer arietinum L.), important pulse (grain legume) crop India. Methods: Seeds were soaked in S, S/2, S/4 extracts rice polish 0.1, 0.3, 0.5% solution pyridoxine for 4 h. Fo...

Meloidogyne incognita and Meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. Microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent Pasteuria penetrans for control of M. incognita on tomato and cucumber and M. arenaria on snapdragon. Three methods of application for P. penetrans were assessed including s...

Cut flower producers currently have limited options for nematode control. Four field trials were conducted in 2006 and 2007 to evaluate Midas® (iodomethane:chloropicrin 50:50) for control of root-knot nematodes (Meloidogyne arenaria) on Celosia argentea var. cristata in a commercial floriculture production field in southeastern Florida. Midas (224 kg/ha) was compared to methyl bromide:chloropic...

Nucleic acid hybridization among root-knot nematode mitochondrial DNAs can be used to identify several Meloidogyne species. Research was initiated to optimize mitochondrial DNA-based molecular diagnostics for the demanding environments likely to be encountered in field isolates. DNA hybridization using reconstituted DNA-soil mixtures revealed a loss of assay sensitivity ranging from 34% to 92% ...

The root-knot nematode Meloidogyne incognita causes severe damage to continuously cropping vegetables. The control of this nematode relies heavily on organophosphate nematicides in China. Here, we described resistance to the organophosphate nematicide fosthiazate in a greenhouse-collected resistant population (RP) and a laboratory susceptible population (SP) of M. incognita. Fosthiazate was 2.7...

Root-knot nematode penetration and morphopathlogy of the infection sites were studied in roots, stem and hypocotyls of Luffa cylindrica, L. and effect of combined inoculation of Vesicular Arbuscular Mycorrhizae (VAM) fungi included Glomus mossae, Glomus intraradices, G. fasciculatum, Acaulospora sp. and Meloidogyne incognita (Root-knot Nematode) were investigated. The maximum number and size of...

Interactions between root-knot nematodes and viruses were recently reviewed by Taylor (4). Compared to virus-free cowpea (vigna sinensis Endl.), cowpea mosaic virus-infected plants formed fewer galls on roots in response to infection by Meloidogyne incognita (Kofoid and White) Chitwood (2). On the other hand, tobacco plants (Nicotiana tabacum L.) infected with tobacco ringspot virus (TRSV) and ...

Sedentary plant-parasitic nematodes are able to induce the redifferentiation of root cells into multinucleate nematode feeding sites (NFSs). We have isolated by promoter trapping an Arabidopsis thaliana gene that is essential for the early steps of NFS formation induced by the root-knot nematode Meloidogyne incognita. Its pattern of expression is similar to that of key regulators of the cell cy...