A simple, 30-mm method of estimating the binding of bilirubin to serum protein was compared with a quantitative method. Good correlation was found between the two methods, both based on Sephadex gel filtration. The results indicate that the presence of loosely bound bilirubin can be determined with this simple, qualitative, semimicro-scale test. This determination is important for the clinical ...

OBJECTIVE The goal was to develop a predictive nomogram, based on transcutaneous bilirubin (TcB) measurements, for assessment of the risk of significant hyperbilirubinemia in healthy term and near-term neonates. METHODS A total of 10382 TcB measurements were performed with 2039 healthy neonates (gestational age of > or =35 weeks and birth weight of > or =2000 g), with a BiliCheck bilirubinome...

Difference spectroscopy is used to monitor bilirubin-protein interactions, to assess the residual binding capacity of proteins for bilirubin. A change in the difference spectra monitored at 482 nm is directly proportional to bound bilirubin up to a molar ratio of bilirubin to albumin of approximately 1; increasing bilirubin beyond the 1:1 molar ratio does not further change the difference spect...

Jaundice is the most common clinical sign in neonatal medicine, but only rarely is it associated with bilirubin neurotoxicity or the harbinger of significant underlying disease. Cases of kernicterus, which should be a never event, are still occurring. Delays in the diagnosis of pathological causes of prolonged jaundice, such as biliary atresia are still resulting in life long morbidity. These a...

INTRODUCTION This study aims to determine the aetiology and complications of exchange transfusion (ET) performed for neonatal hyperbilirubinaemia in Isfahan, Iran. METHODS A retrospective chart review of 68 term and near-term newborns who underwent ET at two perinatal centres in Isfahan, Iran between January 2001 and January 2004, was performed. RESULTS Of the 68 patients who underwent ET, ...

Here we report a severe case of hemolytic anemia of the newborn with kernicterus caused by anti-Di(a) antibody. A full term male infant was transferred due to hyperbilirubinemia on the third day of life. Despite single phototherapy, the baby's total bilirubin had elevated to 30.1 mg/dL. After exchange transfusion, total bilirubin decreased to 11.45 mg/dL. The direct antiglobulin test on the inf...

Neonatal jaundice or hyperbilirubinemia and its evolution to acute bilirubin encephalopathy (ABE) and kernicterus are an important, yet avoidable, origin of newborn deaths, re-hospitalisations and disabilities generally. In this study, a new supervised hybrid bijective soft set neural network-based classification method is introduced for prediction of Egyptian neonatal jaundice dataset. Early p...