Objective Evidence suggests that keratinocyte–fibroblast interactions are abnormal in systemic sclerosis (SSc). The present study was undertaken to investigate potential epidermal dysfunction SSc and its effects on dermal homeostasis. Methods Epidermal equivalents (EEs) were generated using keratinocytes from 6 healthy donors 4 individuals with SSc. Skin EE expression of markers proliferation, ...

The human interfollicular epidermis is renewed throughout life by populations of proliferating basal keratinocytes. Though interfollicular keratinocyte stem cells have been identified, it is not known how self-renewal in this compartment is spatially organized. At the epidermal-dermal junction, keratinocytes sit atop a heterogeneous mix of dermal cells that may regulate keratinocyte self-renewa...

Psoriasis, acne vulgaris and photoaging are common conditions. Tazarotene is a pro-drug of tazarotenic acid, a receptor-selective retinoid, which has shown efficacy in the treatment of these disorders. In the treatment of acne vulgaris, it has greater comedolytic activity than the currently available topical retinoids. In psoriasis, tazarotene normalizes keratinocyte differentiation, reverses k...

An optimum culture condition was established for our keratinocyte cell culture system from human adult skin using 3T3 feeder cells. Calcium ion (Ca(+)+ concentration was found to be critical and cells grew best at the Ca(+)+ concentration of 0.2 mM. Keratinocyte proliferation was promoted when 0.4 micrograms/ml hydrocortisone and 7 micrograms/ml insulin were added. However, epidermal growth fac...

OTULIN is a deubiquitinase that specifically cleaves linear ubiquitin chains. We demonstrate ablation of selectively in keratinocytes, results the appearance inflammatory skin lesions develop into verrucous carcinomas. Genetic TNFR1, knockin expression kinase-inactive RIPK1 or keratinocyte-specific deletion FADD and MLKL completely rescues mice with deficiency from dermatitis tumorigenesis, ide...