Assay of acetylcholinesterase (AChE) activity plays an important role in diagnostic, detection of pesticides and nerve agents, in vitro characterization of toxins and drugs including potential treatments for Alzheimer's disease. These experiments were done in order to determine whether indoxylacetate could be an adequate chromogenic reactant for AChE assay evaluation. Moreover, the results were...

A pipeline has been created for the characterization of Arabidopsis thaliana mutants by generating flanking sequence tags (FSTs) and optimized for economic, high-throughput production. The GABI-Kat collection of T-DNA mutagenized A. thaliana plants was used as a source of independent transgenic lines. The pipeline included robotized extraction of genomic DNA in a 96-well format, an adapter-liga...

SimpleSearch provides access to a database containing information about T-DNA insertion lines of the GABI-Kat collection of Arabidopsis thaliana mutants. These mutants are an important tool for reverse genetics, and GABI-Kat is the second largest collection of such T-DNA insertion mutants. Insertion sites were deduced from flanking sequence tags (FSTs), and the database contains information abo...

Lysine acetyltransferases (KATs) play a critical role in the regulation of transcription and other genomic functions. However, a persistent challenge is the development of assays capable of defining KAT activity directly in living cells. Toward this goal, here we report the application of a previously reported dCas9-p300 fusion as a transcriptional reporter of KAT activity. First, we benchmark ...

Özet Amaç: Baş ağrısı tüm yaş gruplarında sık görülen yaygın bir halk sağlığı problemi olmakla birlikte, yaşa, cinsiyete ve toplum özelliklerine göre farklılık göstermektedir. Bu hastane bazlı çalışmada, Türkiye’nin kırsal bir bölümünde baş ağrısının özellikleri ve alt tipleri cinsiyete ve yaşa göre incelendi. Gereç ve Yöntem: Toplam 11549 hasta değerlendirildi ve 4951 hastada (%42.8) baş ağrıs...

Kleene algebras with tests (KATs) o er sound, complete, and decidable equational reasoning about regularly structured programs. Since NetKAT demonstrated how well various extensions of KATs apply to computer networks, interest in KATs has increased greatly. Unfortunately, extending a KAT to a particular domain by adding custom primitives, proving its equational theory sound and complete, and co...

In the present study, we identified a missense mutation (G199V) in KAT-18 cell line established from primary cultures of anaplastic thyroid cancer (ATC). Notably, knockdown of this mutant (mt) p53 reduced cell viability and exerted antitumor activity equivalent to high doses of several chemotherapeutic agents. We showed that p53 knockdown had an antitumor effect via the induction of apoptosis. ...

In our view, all current software engineering tools and techniques have strengths and weaknesses but very few tools provide much in the way of useful support for the critical “people-related” (or “softer”) organisational behaviour issues critical to the successful implementation of any new process or system. In this context, activity theory would appear to have much to offer, incorporating, as ...