Plasma phosphate values are significantly lower in spontaneously hypertensive rats (SHR) than in normotensive Wistar-Kyoto rats (WKY). In this study, we increased plasma phosphate in SHR by a dietary phosphate intake and followed the effects on blood pressure. Fifteen male WKY and 15 male SHR were housed from 4 weeks of age up to 26 weeks. At 4 weeks of age all SHR manifested a hypophosphatemia...

This paper deals with multiple response simultaneous optimization using the Derringer’s desirability function for the development of a reversed-phase HPLC methods for the simultaneous determination of Ambroxol(AMB) and Montelukast(MLS) with Levocetirizine (LCT) in commercial pharmaceutical preparations with Probenecid(PRO) as Internal standard. The ranges of the independent variables used for t...

Marine endophytic fungi isolated from Red Sea organisms were screened for the production of dextranase enzyme. The most potent isolate was from the Red Sea sponge Callyspongia spp. and was selected for identification. The18S rRNA amplification for phylogenetic study revealed that the isolate was highly related to Aspergillus flocculosus strain NRRL 5224 by 99 %. Medium composition and culture c...

Direct reactions of the MRI contrast agent K2[Gd(DTPA)(H2O)]·5H2O (1) (H5DTPA = diethylenetriaminepentaacetic acid) with dipotassium hydrogen phosphate (K2HPO4) or phosphite (K2HPO3) result in the isolation of well-defined Gd-DTPA phosphite K6[Gd2(DTPA)2(HPO3)]·7H2O (2) or phosphate K6[Gd2(DTPA)2(HPO4)]·10H2O (3), respectively. Their lanthanum analogs K4[La2(DTPA)2(H2O)]·8H2O (4), K6[La2(DTPA)2...

Statistical experimental design was employed to optimize medium compositions to enhance the activity of lipase from Bacillus subtilis. Plackett–Burman design was applied to evaluate the effect of different medium compositions obtained by one-factor-at-a-time experiments, and ammonium chloride, ammonium sulfate and dipotassium hydrogen phosphate were found to influence the activity of lipase sig...

The objective of this investigation was to develop a new auxotropic mutant from a regulatory mutant and to examine its potency for L-lysine production. A high L-lysine yielding strain Micrococcus glutamicus AB200 was developed from a regulatory mutant Micrococcus glutamicus AB5 by subsequent mutagenic treatment with UV rays and methyl methane sulfonate respectively. Treatment of UV rays on the ...

Sir: We have established a screening system for differentiation inducing substances of animal cells1). Using this system, we have recently isolated a new substance from culture filtrate of a streptomycete, which induces differentiation of mouse leukemia cells. The active substance was named differenol A. Streptomyces sp. ANS-127, the differenol Aproducing organism was isolated from a soil sampl...

A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease. Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour. Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was eva...

BACKGROUND The effectiveness of bioremediation systems for PAH-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. Information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist the biodegradation efficacy. METHODS In this study, four factors of surfac...

Pd-catalyzed carboxylic acid guided regioselective alkynylation of cage B(4)–H bonds in o-carboranes has been achieved for the first time using two different catalytic systems. In the presence of 5 mol% Pd(OAc)2 and 3 equiv. of AgOAc, the reaction of 1-COOH-2-R-C2B10H10 with R3SiC^CBr in ClCH2CH2Cl gives 4(R3SiC^C)-2-R -o-C2B10H10 in moderate to high yields. This reaction is compatible with alk...