partial frames provide a rich context in which to do pointfree structured and unstructured topology. a small collection of axioms of an elementary nature allows one to do much traditional pointfree topology, both on the level of frames or locales, and that of uniform or metric frames. these axioms are sufficiently general to include as examples bounded distributive lattices, $sigma$-frames, $ka...

This paper presents a POCS-based algorithm for consistent reconstruction of a signal x 2 R from any subset of quantized coe cients y 2 R in an N K overcomplete frame expansion y = Fx, N = 2K. By choosing the frame operator F to be the concatenation of two K K invertible transforms, the projections may be computed in R using only the transforms and their inverses, rather than in the larger space...

In this paper a new graph partitioning problem is introduced, the relaxed k-way graph partitioning problem. It is close to the k-way, also called multi-way, graph partitioning problem, but with relaxed imbalance constraints. This problem arises in the air traffic control area. A new graph partitioning method is presented, the Fusion Fission, which can be used to resolve the relaxed k-way graph ...

PURPOSE EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. EXPERIMENTAL DESIGN Primers were designed to detect a...

Herpes simplex virus type 1 (HSV-1) syncytial (syn) mutants cause formation of giant polykaryocytes and have been utilized to identify genes promoting or suppressing cell fusion. We previously described an HSV-1 recombinant, F1 (J.L. Goodman, M. L. Cook, F. Sederati, K. Izumi, and J. G. Stevens, J. Virol. 63:1153-1161, 1989), which has unique virulence properties and a syn mutation in the carbo...

More than 20 different partner genes with MLL have been cloned from leukemia cells with translocations involving chromosome 11 band q23 (11q23). All reported partner genes fused in-frame to MLL and the fusion cDNA encode chimeric MLL proteins with a significant portion derived from the partner genes. We analyzed one patient with de novo acute monoblastic leukemia with t(11;14)(q23;q24) and iden...

A plasmid was constructed that facilitates the cloning and expression of open reading frame DNA. A DNA fragment containing a bacterial promoter and the amino terminus of the cI gene of bacteriophage lambda was fused to an amino-terminally deleted version of the lacZ gene. An appropriate cloning site was inserted between these two fragments such that a frameshift mutation was introduced upstream...

In this paper we investigate the modulation domain as an alternative to the acoustic domain for speech enhancement. More specifically, we wish to determine how competitive the modulation domain is for spectral subtraction as compared to the acoustic domain. For this purpose, we extend the traditional analysis-modification-synthesis framework to include modulation domain processing. We then comp...

Fusion of the anaplastic lymphoma receptor tyrosine kinase gene (ALK) with the echinoderm microtubule-associated protein 4 gene (EML4) is the second most common actionable alteration in non-small-cell lung cancer, with a frequency of 5%. Here, we present a case of an EML4-ALK-positive patient with an atypical in-frame insertion from the LTBP1 gene in the canonical junction of variant 1. The pat...

We have cloned and determined the nucleotide sequence of the prlF gene. An open reading frame predicting a 111-amino-acid protein (Mr 12,351) with an acidic carboxy terminus was identified. The DNA sequence preceding this open reading frame revealed a putative promoter and a ribosome-binding site. The nucleotide sequence of the prlF1 mutation revealed a 7-base-pair duplication resulting in a sl...