BACKGROUND RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. METHODS Small populations of K562 cells or PMBC were sorted into the lysing reagent and subjected to RNA extraction, employing eith...

Marine sediment represents one of the most significant carbon reservoirs. Extraction pore water from uppermost 10 cm column, just below sediment-water interface, in coastal environments can be used to assess rapid chemical changes caused by extensive subsurface biological and reactions. However, owing relatively large grain size some shallow environments, mixes quickly making collection core sa...

A study on the extraction of Acetaminophen (ACTP) which is also known as paracetamol, from aqueous solution by emulsion liquid membrane process using Taylor-Couette Column (TCC) was investigated. An ELM consists of three phase system which are the external, membrane and internal phases. The external phase containing the ACTP aqueous solution to be treated. Basically, the internal and membrane p...

An experimental study of the phase inversion behavior of liquid-liquid dispersion has been conducted in a spray extraction column for systems of toluene / water, n-hexane/water, CCl4/water, toluene /water + glycerol (25 % wt), toluene + CCl4 (25 % wt) / water and toluene / acetic acid (5 % wt)/water. The effects of physical properties, mass transfer and column geometry...

In the present investigation the biosorption potential of rosa gruss an teplitz (Red Rose) Waste Biomass (RRWB) for the removal of Pb(II) and Co(II) from aqueous solutions was studied. The effect of different process parameters such as pH, biosorbent dose, biosorbent size, temperature, contact time, initial metal concentration and pretreatments on the biosorption capacity of this waste biom...

Objective(s):The present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. Materials and Methods:The HPLC method was carried out by using a C18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the flow...

Objective(s) A sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) is described. Materials and Methods The method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes...