Due to the problems encountered in routine morphological and physiological procedures that are used in yeast identification, DNA-based methods have recently been developed. In the present study, l66 yeast strains were isolated from several apple and citrus cultivars. After analysis by basic morphological methods, the ITS1 and ITS2 regions of the isolates were amplified separately, and the isola...

background and aims: plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. reverse transcriptase-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certification of plant propagating materials. the main object of the present study was the optimization ...

background: toxoplasma gondii is an intracellular parasite, which infects one-third population of world. humans and animals acquire infection by ingesting oocytes from feces of cats or by meat of other animals having cysts that may lead to congenital, ocular or cephalic toxoplasmosis. either it is important to detect t. gondii from meat of food animals from retail shops or directly at slaughter...

Abstract. Dami JC, Damayanti LPE, Indarjulianto S, Priyowidodo D. 2023. Ancylostomiasis in cats Yogyakarta, Indonesia, and its causative genetic relations. Biodiversitas 24: 2605-2611. is a zoonotic cat disease caused by one or more species of Ancylostoma spp. This study aims to determine ancylostomiasis Yogyakarta research used ten with ancylostomiasis, based on the findings eggs their feces. ...

BACKGROUND Second internal transcribed spacer (ITS2) has proven to contain useful biological information at higher taxonomic levels. OBJECTIVES This study was carried out to unravel the biological information in the ITS2 region of An. culicifacies and the internal relationships between the five species of Anopheles culicifacies. METHODOLOGY In achieving these objectives, twenty two ITS2 seq...

Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDN...

ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, has often been linked to oral transmission through açai consumption. Molecular methods that allow fast and accurate identification of pathogen are important for detection presence parasite in this food. This study aimed optimize polymerase chain reaction (PCR)-based T. cruzi DNA pulp. Several dilutions DTU TcI trypomastigote fo...

background: infectivity of herbivores with trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. this study was carried out on trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification. methods: trichostrongylus isolate...

The nuclear ribosomal Internal Transcribed Spacer ITS region is widely used as a DNA metabarcoding marker to characterize the diversity and composition of fungal communities. In amplicon pyrosequencing studies of fungal diversity, one of the spacers ITS1 or ITS2 of the ITS region is normally used. In this methodological study we evaluate the usability of ITS1 vs. ITS2 as a DNA metabarcoding mar...