نتایج جستجو برای: its1 fragment
تعداد نتایج: 63679 فیلتر نتایج به سال:
We introduce an open source software utility to extract the highly variable ITS1 and ITS2 subregions from fungal nuclear ITS sequences, the region of choice for environmental sampling and molecular identification of fungi. Inclusion of parts of the neighbouring, very conserved, ribosomal genes in the sequence identification process regularly leads to distorted results. The utility is available ...
Abstract The present study aimed to evaluate a methodology for active surveillance of visceral leishmaniasis by detecting Leishmania DNA in organs wild road-killed animals from November 2016 October 2018 the North Paraná, Brazil. collection points were georeferenced. autopsied and samples bone marrow, lymph node, liver, spleen, ear skin collected. Genomic was extracted subjected PCR amplificati...
Three PCR assays for diagnosing leishmaniasis were compared and validated against parasite cultures and microscopic evaluation of stained tissue smears using 92 specimens from suspected cases of cutaneous leishmaniasis (CL) in Israel and the West Bank. Samples from imported and locally acquired disease were examined. The kinetoplast DNA (kDNA) PCR showed the highest sensitivity (98.7%) of any a...
A total of 134 Egyptian liver flukes were collected from different definitive hosts (cattle, sheep, and buffaloes) to identify them via the use of PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1). Specimens of F. hepatica from France, as well as F. gigantica from Cameroon were included in the study for comparison. PCR products of ITS1 were subject...
Kefir has attracted interest in the scientific community due to its beneficial properties, such as: improving digestion, antimicrobial action, hypocholesterolemic, antihypertensive, and anti-inflammatory effects, control of plasma glucose levels, antioxidant, anticarcinogenic, antiallergic activity. The comparative characteristics microbiomes kefir grains (KG) obtained from regions their histor...
Polygalaceae is a large family with cosmopolitan distribution, comprising ca. 1,200 species in 27 genera. However, similar to many plant groups, the identification of mostly depends on floral and fruit characteristics; therefore, DNA barcoding could easily warrant corect sterile material. In current study, utility six widely employed barcode loci, namely rbcL, matK, trnL-F region (including trn...
زمینه و هدف: لیشمانیا ماژور و لیشمانیا تروپیکا عوامل اصلی به وجود آورنده لیشمانیوز جلدی در بخش های مختلف ایران بویژه دو منطقه اصفهان و بم می باشند. در این مطالعه به دلیل تأثیر تنوع سویه های انگل در طراحی استراتژی های کنترل بیماری، ایزوله های انسانی جهت تعیین نوع سویه با استفاده از روش pcr-rflp مورد بررسی قرار گرفتند. مواد و روش ها: در این مطالعه تجربی از 340 گونه ی جدا شده از بیماران دارای زخم ...
Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used cultivation Asia, America Europe are available The present study investigated indigenous hardwoods their supplementation with rice wheat bran as substrates its cultivation. Six (Mansonia altis...
background: cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and iran. since extraction of dna from the parasite is a primary and crucial step which has a principal effect on pcr results, in the current study five simple methods for dna extraction from protoscoleces of echinococcus granulos...
Two new species isolated from plant leaves belonging to Talaromyces section Talaromyces are reported, namely T. neofusisporus (ex-type AS3.15415 (T) = CBS 139516 (T)) and T. qii (ex-type AS3.15414 (T) = CBS 139515 (T)). Morphologically, T. neofusisporus is featured by forming synnemata on CYA and YES, bearing appressed biverticillate penicilli and smooth-walled fusiform conidia about 3.5-4.5 × ...
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