Previous studies (1) have indicated that a-GP’ is an important intermediate in the incorporation of radioactive inorganic phosphate into a phosphatide fraction of rat liver. The dependence of the incorporation on oxidative phosphorylation and the marked stimulation by the addition of free glycerol suggested that the first stages in this reaction sequence were the conversion of radioactive ortho...

It has been shown in a preceding paper (I) that glycine in liver and kidney cells is metabolically inhomogeneous for short time intervals. Intravenously administered radioactive glycine was trapped in a synthetic reaction and found to have greater specific activity than the total cellular glycine recovered simultaneously. The reaction used, hippurate synthesis, is a mitochondrial reaction (2, 3...

The energized uptake of low levels of Ca(2+) in the presence and absence of phosphate by isolated rat liver mitochondria, and the perturbation effected by this activity on ultrastructural and metabolic parameters of mitochondria have been investigated. In the presence of phosphate, low levels of Ca(2+) are taken up by mitochondria and result in various degrees of ultrastructural expansion of th...

Initial rates of [3H]adenosine diphosphate and [3H]adenosine triphosphate uptake were measured in mitochondria isolated from normal rat liver, regenerating liver, mouse hepatoma BW7756, and four Morris hepatomas (7777, 7800, 7794A, and 16) of varying degrees of malignancy. Results obtained demon strate that (a) the apparent Km and Vmaxvalues for adenosine diphosphate and adenosine triphosphate ...

Initial rates of [3H]adenosine diphosphate and [3H]adenosine triphosphate uptake were measured in mitochondria isolated from normal rat liver, regenerating liver, mouse hepatoma BW7756, and four Morris hepatomas (7777, 7800, 7794A, and 16) of varying degrees of malignancy. Results obtained demon strate that (a) the apparent Km and Vmaxvalues for adenosine diphosphate and adenosine triphosphate ...

Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the mono...

The production of ATP is vital for muscle contraction, chemiosmotic homeostasis, and normal cellular function. Many studies have measured ATP content or qualitative changes in ATP production, but few have quantified ATP production in vivo in isolated mitochondria. Because of the importance of understanding the energy capacity of mitochondria in biology, physiology, cellular dysfunction, and ult...

A constant morphological feature of normal mitochondria, either isolated or in the intact tissue, is the presence of varying numbers of dense granules in the matrix. Isolated mitochondria contain relatively large amounts of Ca + + and Mg ++ (9) and are also able to accumulate divalent cations (1-3). Peachey (6) suggested that the normal matrix granules prime the accumulation of divalent cations...

In chronic glucagon-treated ducklings (GT) showing thermogenic and hyperthermic responses without shivering to glucagon test injection and in control ducklings (TN; both aged 44 +/- 1 days and reared at thermoneutrality), subsarcolemmal (S) and intermyofibrillar (I) mitochondria from gastrocnemius muscle and mitochondria from liver were isolated. Respiration and cytochrome oxidase activity were...