A simple stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of diclazuril (DIC) in the presence of its induced degradation products. The drug was subjected to stress stability study including acidic, alkaline, photolytic, thermal and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. The ...

A simple, specific and reproducible liquid chromatography-electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was achieved on a Venusil MP-C18 column using an isocratic elution. Jolkinolide B and osthole (internal standar...

In this paper we describe a rapid, isocratic high performance liquid chromatography (HPLC) method for the study of radioactive fatty acid incorporation into complex lipids of human erythrocytes, which allows the simultaneous separation of the major phospholipid classes and long-chain acylcarnitines. The lipid extract of erythrocytes pulsed with radioactive fatty acids was injected into an HPLC ...

This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a fl...

A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine, were extracted from plasma matrix by liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (V/V)...

A simple, selective, precise and accurate stability-indicating high-performance liquid chromatographic method for analysis of Erdosteine and its degradation products in tablet dosage form was developed and validated. The stability indicating ability of the method was determined by performing forced degradation studies which indicated susceptibility of the drug under acidic and neutral hydrolyti...

A rapid, specific, and reliable LC-MS/MS-based bioanalytical method was developed and validated in rat plasma for the simultaneous quantitation of amitriptyline and its metabolite nortriptyline. Chromatographic separation of these analytes was achieved on a Gemini C18 column (50 × 4.60 mm, 5 μm) using reversed-phase chromatography. The mobile phase was an isocratic solvent system consisting of ...

The concentrations of amoxicillin in pig plasma were determined by HPLC technique. Separation was achieved on a Lichrocart 250 x 4 mm Purospher column and detection was performed with a fluorescence detector. The mobile phase consisted of a mixture of acetonitrile with buffer solution (0.05 M KH2PO4, pH 5.6) delivered by a isocratic programme. For amoxicilin assay, the plasma samples were expos...

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of amlodipine and clopidogrel in table formulation. The separation and quantification was achieved by thermo hypersil BDS C18 250x4.6x5mm column in isocratic mode, with mobile phase consisting of buffer-methanol(30:70v/v). The mobile phase was pumped at a rate of 1.5 ml/min and the detection ...

A simple stability indicating reversed-phase HPLC method was developed and subsequently validated for estimation of Cefpirome sulphate (CPS) present in pharmaceutical dosage forms. The proposed RP-HPLC method utilizes a LiChroCART-Lichrosphere100, C18 RP column (250 mm × 4mm × 5 μm) in an isocratic separation mode with mobile phase consisting of methanol and water in the proportion of 50:50 % (...