A method for the measurement of uric acid in human serum by isotope dilution-mass spectrometry is described. The analytical procedure consists of the following steps: Addition of [1,3-15N2]uric acid to the serum sample; ion exchange chromatography on AG1-X2; formation of the trimethylsilyl derivative; gas liquid chromatography-mass spectrometry (GC-MS), selected ion monitoring (SIM) at m/z-valu...

Corresponding Author: Zhiwei Chen School of Life Sciences, Shandong University of Technology, 255049, Zibo, China Tel: +86-05332786781, Fax: +86-05332786781 Email: [email protected] Abstract: In this study, the anions nitrite ion (NO2 ) and nitrate ion (NO3 ) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the c...

a rapid isocratic method of high performance liquid chromatography system (hplc) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (no2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. the solid extraction  reversed phase cartridges (sep-pak) are used for dep...

Abstract The paper presents a cascade of preprocessing procedures aiming at enhancing the quality images generated by gas-chromatography ion mobility spectrometers (GC-IMS). methods successfully eliminate Reactant Ion Peak (RIP) lines, while additionally filtering out noisy components. resulting enable better localization and matching peaks associated with presence distinct analytes in samples ...

The methods employed previously in this laboratory for the isolation of oxytocin and vasopressin depend upon the use of several procedures to arrive at a highly purified sample. From the acetone powder of the posterior pituitary gland, the extraction and fractionation method of Kamm and coworkers (1) leads to a fraction rich in oxytocin and one rich in vasopressin, each at a stage of intermedia...

Glyphosate is widely used in herbicides and is toxic to organism. Therefore accurate detection of glyphosate content is important for public health and food security. In this paper, methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC/MS), liquid chromatography-mass spectrometry (LC/MS), ion chromatography (IC), capilla...

Abbreviations: FVIII/vWF: Complex of Factor VIII and von Willebrand Factor; MW: Molecular Weight; NDF: Number of Degree of Freedom; S/D: Solvent/Detergent; LEV/LEVs: Virus (es) Enveloped by Lipid Membrane; NLEV/NLEVs: Non-enveloped Virus (es); IFA method: Method of Immune-fermental Analysis; FITC: Fluorescein Isothiocyanate; RT-PCR method: Real-time PCR Method; TCID50: 50% Tissue Culture Infect...

Antibodies are a class of biomolecules that has an important role in the immune system and lots of applications in biotechnological methods and in pharmaceutics. Production and purification of antibodies in laboratory animals is one of the first ways to manufacture of these prominent tools. The obtained antibodies from these process could be used in various types of bioassay techniques such as ...

Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a the...