O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-manno...

Using pea as our model crop, we sought to understand the regulatory control over import of sugars and amino acids into developing seeds its importance for seed yield quality. Transgenic peas simultaneously overexpressing a sucrose transporter an acid were developed. Pod walls, coats, cotyledons analysed separately, well leaves subtending pods. Sucrose, starch, protein, free acids, endogenous cy...

Investigation various nitrogen sources effects on the production of invertase by Aspergillus niger was researched in this study. Invertase is a precious enzyme used many industries like food, pharmacy, confectionery, invert syrup production. Taguchi design experiment (DOE) preferred to optimize cultivation conditions. L16 (43) orthogonal array selected current study including source, initial pH...

Mutations in the SNF2 gene of Saccharomyces cerevisiae prevent derepression of the SUC2 (invertase) gene, and other glucose-repressible genes, in response to glucose deprivation. We have isolated 25 partial phenotypic revertants of a s n . mutant that are able to derepress secreted invertase. These revertants all carried suppressor mutations at a single locus, designated SSN20 (suppressor of sn...

Invertase inhibitors have been isolated and partially purified from red beets, sugar beets, and sweet potatoes. These inhibitors are thermolabile proteins with molecular weights of 18,000 to 23,000. They do not inhibit yeast and Neurospora invertases, but they are reactive with potato tuber invertase and other plant invertases with pH optima near 4.5. There are differences in reactivity of the ...

A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by actinomycin D, cycloheximide, and glycerol. The starving cells of the mutant st...

The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-pho...