The gene encoding the E subunit (atpf?) of the chloroplast ATP synthase of Spinacia oleracea has been overexpressed in Escherichia COK The recombinant protein can be solubilized in 8 M urea and directly diluted into buffer containing ethanol and glycerol to obtain E that i s as biologically active as E purified from chloroplastcoupling factor 1 (CF,). Recombinant E folded in this manner inhibit...

Four mechanisms by which peritrophic membranes (PMs) potentially protect herbivorous insects from ingested allelochemicals are reviewed: adsorption, ultrafiltration, polyanion exclusion, and the capacity of PMs to act as antioxidants. Most of the research on the protective roles of PMs against ingested allelochemicals has focused on their impermeability to tannins. Adsorption of tannins by the ...

The impermeability of concrete the exterior wall underground tunnel in water-rich stratum is a key concern engineers. Taking Aixihu highway Nanchang city as an example, side tested, through multiple tests, such embedded steel pipe, water injection, and field observation. results show that, under action 2mpa pressure, no flow permeates from tunnel, indicating that impermeable performance this se...