Dramatic changes in morphology and extensive reorganization of membrane-associated actin filaments take place during mitosis in cultured cells, including rounding up; appearance of numerous actin filament-containing microvilli and filopodia on the cell surface; and disassembly of intercellular and cell-substratum adhesions. We have examined the distribution and solubility of the membrane-associ...

A new three-dimensional unit cell model has been developed for modeling three constituent phases including inclusion, interphase and matrix. The total elastic modulus of nano-composite is evaluated.  Numerical results are in good agreement with the previous proposed theoretical modeling. Higher matrix and inclusion elastic modulus both can dramatically influence the total elastic modulus.

Evanescent wave spectroscopy using thin optically transparent electrodes and multiple reflections allows unique observations of the electrochemical interphase since the region of solution sampled is typically 100 nm from the electrode in the visible spectral region. This technique is found to be highly sensitive to the presence of absorbing adsorbed species

Given the time-consuming and complex nature associated with aging of composites, a novel fabric pre-aging method was developed evaluated for static fatigue testing. It allows investigating sizing interphase-related effects. This fast is independent diffusion processes composites’ thickness. Moreover, new methodology offers enhanced analysis sizing, interphase, fiber-related degradation composit...

Heat denaturation of DNA in situ, in unbroken cells, was studied in relation to the cell cycle. DNA in metaphase cells denatured at lower temperatures (8~176 lower) than DNA in interphase cells. Among interphase cells, small differences between G1, S, and G2 cells were observed at temperatures above 90~ The difference between metaphase and interphase cells increased after short pretreatment wit...

The objective of this study was to design DNA probe sets that enable the detection of chromosome aberrations in acute myeloid leukemia (AML) by interphase cytogenetics using fluorescence in situ hybridization (FISH) and to compare the results of interphase cytogenetics with those of conventional chromosome banding analysis. One hundred five consecutive patients with adult AML entered on a multi...

We have developed a new method for determining levels of tubulin polymer, based on quantitative fluorescence detection of x-rhodamine tubulin microinjected into living cells and we have applied this method to analysis of the mitosis-interphase transition. LLC-PK cells in interphase and mitosis were microinjected, then cooled and rewarmed to drive tubulin incorporation. Total tubulin fluorescenc...

In interphase HeLa cells, incubation with histamine or thapsigargin led to the rapid release of arachidonic acid. The release was absolutely dependent on Ca2+, consistent with the activation of an 85 kDa cytosolic phospholipase A2 (cPLA2). In metaphase-arrested HeLa cells, by contrast, the stimulation of arachidonate release by these agents was inhibited by more than 90%. The lack of arachidoni...