To evaluate the age-dependent phosphate homeostasis, we studied the serum inorganic phosphate (P(i)) concentration in 78 recipients, aged 5-25 years, a year after renal transplantation (RT). The significant age-dependent decline of the serum P(i) concentration was observed in recipients (p < 0.0001) as well as in normal children. Our study revealed that a circulating factor may play a central r...

* Predoctoral Fellow of the National Science Foundation, 1954-55; present address, Walter Reed Army Institute of Research, Washington 12, D. C. This investigation was supported by grants from the National Institutes of Health, Public Health Service, and the National Science Foundation. 1 Abbreviations used: ATP, adenosine triphosphate; 05P, orotidine 5’-phosphate; PP, inorganic pyrophosphate; P...

Phosphorus contents of epidermal vacuoles and mesophyll cells of Lupinus lutens leaflets were measured by electron probe x-ray microanalysis of fully hydrated, bulk frozen samples. Quantitation was achieved using standard solutions containing colloidal graphite to simulate cell contents and the derived nonlinear relationship between peak over background ratio and concentration improved the accu...

The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medi...

The reaction of nicotinamide mononucleotide (nicotinamide ribose phosphate) with adenosinetriphosphate (ATP), catalyzed by a specific enzyme in liver and yeast, has been shown to result in the production of diphosphopyridine nucleotide (DPN) and inorganic pyrophosphate (PP) (1). Similarly, the reaction of riboflavin phosphate with ATP, catalyzed by another yeast enzyme, produces flavin-adenine ...

The asymmetric unit of the title compound, [Zn(HPO(4))(C(2)H(3)N(3))](n), contains one Zn(2+) cation, one (HPO(4))(2-) anion and a 1,2,4 triazole ligand. The Zn(2+) cation is coordinated in a quite regular tetra-hedral geometry by O atoms from three phosphate groups and a tertiary N atom from the triazole ring. Each phosphate anion is connected to three Zn(II) cations, leading to a series of co...

Apyrases are enzymes that efficiently hydrolyze ATP and ADP and may operate both inside and outside the cell. Although apyrases are important to a variety of cellular mechanisms and uses in industry, there are no available apyrase-specific inhibitors. Colorimetric assays based on the Fiske-Subbarow method for measuring inorganic phosphate are able to detect the release of inorganic phosphate fr...

Inorganic phosphate, glucose l-phosphate, divalent metal ions, EDTA, m-propoxybenzamidine, and AMP were found to be competitive inhibitors of the reaction catalyzed by phosphorylase phosphatase. By a comparison of kinetic studies with the substrates, phosphorylase a and the phosphorylated peptide, it was deduced that inorganic phosphate affected the reaction by binding to the catalyst, whereas ...