An extracellular enzyme from culture filtrates of Sporotrichum (Chrysosporium) thermophile (A.T.C.C. 42 464) after growth on cellulose or cellobiose was shown to oxidize cellobiose to cellobionic acid in vitro. Lactose and cellodextrins were also efficiently oxidized, but the enzyme was not active against most mono- and di-saccharides. Several redox substances could act as electron acceptors, b...

It has been recently reported that catalytic quantities of crystalline catalase, in the presence of hydrogen peroxide, can rapidly oxidize a variety of molecules larger than hydrogen peroxide and aliphatic alcohols (1). The qualitative experiments have shown that oc-naphthol and p-phenylenediamine are condensed by oxidative coupling to indophenol purple; p-aminobenzoic acid, sulfathiazole, adre...

Three new laboratory tests are currently available for the identification of Pseudomonas aeruginosa isolated from clinical specimens. None of these procedures are specific for P. aeruginosa and cannot be used to differentiate between species within the genus. However, since P. aeruginosa is the only known human pathogen it is the only member of this genus that usually will be encountered in cli...

A simple procedure using an identical manifold was developed for determination of nitrogen (protein) phosphorus, calcium, chloride, copper, manganese, iron, and zinc in feeds and feedstuffs. By changing appropriate reagents and detection wavelength, these 8 elements were determined successively with a simple identical double-line flow injection (FI) manifold. Fl spectrophotometric determination...

It is well known that the determination of ascorbic acid in urine by titration with 2,6dichlorophenol indophenol in acid solution is not a specific method. Various interfering substances have been reported, such as sulfhydryl compounds (l), thiosulfate (2), and pyridium (medication) (3). The same limitations apply to other oxidation-reduction methods, except when the reducing substance is deter...

Purified aconitase, an iron-sulfur protein, from either beef heart mitochondria or pig heart can be activated fully by light when combined with washed thylakoid membranes from pea (Pisum sativum L.) chloroplasts. The light activation of the enzyme does not require any other additive or cofactor and is sensitive to 3-(3,4-dichlorophenyl)-1,1-dimethyl urea, 2,6-dichlorophenol-indophenol, ferricya...

This paper describes a simple flow-injection (FI) manifold for the determination of a variety of species in industrial water. The chemical systems involved in the determination of ammonia (formation of Indophenol Blue), sulfate (precipitation with Ba(II)), and iron (complexation with 1,10-phenanthroline with the help of a prior redox reaction for speciation) were selected so that a common manif...

The requirement for plastoquinone in the photosynthetic phosphorylation activity of spinach chloroplasts has been indicated in an earlier publication (1). Removal of endogenous plastoquinone from lyophilized chloroplasts by heptane extraction caused a loss of photophosphorylation activity. The photophosphorylation activity could be partially restored by addition of pure plastoquinone to the ext...

A protein kinase activity from spinach chloroplasts, tightly associated with the thylakoid membranes, has been solubilized and partially characterized. This membrane-bound protein kinase is stimulated by light and electron transport activity through photosystem II appears to be required for stimulation.Electron transport inhibitors like 3,4-dichlorophenyl-1,1-dimethylurea, Tris, and NH(2)OH tre...