An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l BAP and 0.1 to 0.5 mg l NAA. The optimized callus induction occurred at the concentration of 1.0 mg l BAP and 0.3 mg l NAA. After initiation of callus, it was immediately transferred to MS mediu...

In vitro budinduction and shoot regeneration of Lantana camara L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds...

An efficient protocol was achieved for rapid propagation of Wedelia chinensis (Osbeck) Merr. through axillary bud proliferation and ex vitro rooting. Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 8.87 microM) and indole-3-butyric acid (IBA; 2.46 microM) was optimal for axillary bud proliferation, which developed a mean of 8.3 shoots/node. Excision and culture of node segm...

Tea (Camellia sinensis L.) is a popular world beverage, and propagation of tea plants chiefly depends on the formation of adventitious roots in cuttings. To better understand potential mechanisms involved in adventitious root formation, we performed transcriptome analysis of single nodal cuttings of C. sinensis treated with or without indole-3-butyric acid (IBA) using the Illumina sequencing me...

This study evaluated the use of splice grafting as a propagation strategy for watermelon. In experiment 1, treatments consisted sucrose, antitranspirant A, B, auxin (indole-3-butyric acid (IBA)) at two concentrations (10 and 20 mg·L−1), plus water control. The survival (%) splice-grafted watermelon plants differed due to number days after treatment (p < 0.0001, both). At 21 grafting, treated...

organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of mission and kroneiki cultivars. different parts of embryos were cultures in omc medium containing growth regulators, such as indole butyric acid (iba) and 2-isopentenyl adenine (2-ip) or iba and naphthalene acetic acid (n...

studies have shown that rooting substrate is one of the effective factors at rooting of hard rhizogenetic plant such as henna. the purpose of this study is to determine an appropriate concentration of naphthalene acetic acid (naa), indole butyric acid (iba) and salicylic acid (sa) on rooting of henna. present study showed that there was a great variation in most of the measured characters at p<...

Strigolactones (SLs) are synthesized in roots and control plant development. As phytohormones, SLs regulate architecture, including roots. Recently, the inhibiting effects of on adventitious rooting have been identified. Olive (Olea europaea L.) is consumed for oil table Mediterranean countries an economically important crop. Turkey one with highest olive production. has mostly propagated asexu...

An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige Skoog media supplemented with 3% sucrose, 0.8% agar, various concentrations indole-3-butyric acid (1 to 10 mg/L). Cultures maintained 4 5 weeks at standard conditions allow root induction elongation. Shoot triggered upon...

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 · 2H2O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and...