Sperm chromatin/DNA damage can be measured by a variety of assays. However, it has been reported that these tests may lose prognostic value in Assisted Reproductive Technology (ART) cycles when assessed in post-prepared samples, possibly due to the normalizing effect promoted by sperm preparation procedures. We have recently implemented a modified version of the Diff-Quik staining assay that al...

Objective: To investigate the effect of sperm DNA fragmentation index (DFI) on the clinical outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods: Infertile couples were prospectively recruited in the Reproductive Center in Jiaozuo Maternal and Child Care Service Centre in Henan, China, from July 2015 to December 2016. All the recruited 228 infertile coup...

BACKGROUND Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a rol...

background: luteal phase support is mandatory in art (assisted reproductive technologies) for optimizing outcome, so the luteal phase is supported with either progesterone, addition of estradiol to progesterone, hcg or gonadotropin releasing hormone (gnrh) agonists. supplementation of luteal phase with progesterone is prescribed for women undergoing routine ivf treatment. objective: to compare ...

background: caffeine increases the camp production that stimulates spermatozoa movement. caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization. objective: the aim of this study was to assess the effect of caffeine on sperm's motility, vitality and laboratory fertilization rates in mouse in two t6 and m16 media. m...

background:   case: we discuss 3 clinical cases of tubo-ovarian abscess in women with endometriosis after oocyte retrieval despite antibiotic prophylaxis between 2004 and 2011 at our center, and discuss our experience in the context of earlier reports.   conclusion: it is unclear whether antibiotic prophylaxis is necessary in these women, and which antibiotic is best. only douching with povidon...

background: the selection of blastocyst warmed for transfer is based on pre-freeze morphology in vitrified-warmed single blastocyst transfer cycles. but, it is controversial which parameter of blastocyst morphology most closely related to the clinical outcomes. objective: to estimate the effect of blastocoele expansion, trophectoderm (te) morphology grade, and inner cell mass (icm) morphology g...

Assisted reproductive technologies (ART) for equine, such as in vitro oocyte maturation (IVM) and fertilization (IVF), has been slow with low success rates compared to other domestic species [1]. This is partly because mares are long-lived animals that gelded at an early age management [2].

This paper introduces a new IV strategy based on IVF (in vitro fertilization) induced fertility variation among childless women to estimate the causal effect of having children on their career. For this purpose, we use administrative data on IVF treated women in Denmark. Because observed chances of IVF success do not depend on labor market histories, IVF treatment success provides a plausible i...

Background: Effect of different doses of cysteamine on rate of in vitro maturation (IVM), in vitro fertilization (IVF) and glutathione (GSH) level was studied. Metaphase II (MII) spindle area was analyzed for quantification of shape and size of oocytes. Methods: Female mice were primed with 5 IU of pregnant mare’s stimulating gonadotrophin. Germinal vesicle (GV) oocytes were retrieved 48 hrs la...