background: progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period. objective: the objective of this study was to evaluate the effect of various concentrations of myo-inositol during in vitro fertilization of bovine oocytes on subsequent embryo development. materi...

background: all-trans retinol is a biological antioxidantscavenging the ros in the cell culture. objectives: this studywas conducted to investigate the effect of all-trans retinol infertilization and culture medium on mouse embryo's developmentalcompetence. methods: this study was designed into twoexperiments. in the first experiment, in vitro mature oocytes wereco-cultured with sperm in fertil...

Oocyte quality is the resultant of multifactor interactions that should be carefully taken into account either for in vitro embryo production technologies or for studying follicle dynamics. Different approaches may be used to perform an analysis of variables related to oocyte quality which may focus on the ovary, the follicle, the cumulus-oocyte complex and, finally, the oocyte. The information...

Conflicting data still exist regarding the extent of paternal pronuclear DNA demethylation in one cell-stage mammalian embryos. Demethylation of paternal pronuclear DNA was observed in in vivo produced porcine zygotes, whereas in vitro produced embryos do not show any or only weak paternal genome demethylation. In our experiments, we have used and compared two in vitro techniques commonly used ...

Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...

flame seedless is one of the most important and commercial table grape cultivar that is used for breeding program due to its specific characteristics of fruit high production. in order to breed new seedless grape cultivars and with considering importance of selecting seedless varieties as a female parent to increase the production rate of seedless offspring in a breeding cycle, embryo rescue te...

The study was conducted to compare differences in vitro maturation media and their effect on subsequent embryo production. procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) Tshwane university of technology AREC 2021/08/004). Follicular fluid containing a suspension oocytes retrieved from ovaries through aspiration slicing techniques. g...