The natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. Optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. In order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

Proliferation kinetics and cell cycle parameters have been measured in exponentially growing and density-inhibited (daily medium changes) stable-plateau phase cultures of Chang human liver (LICH) cells. The mean duration of all phases of the cell cycle among the cycling population was approximately doubled in density-inhibited cultures. About 50% of the population was effectively noncycling but...

background: based on recent studies, there are controversial reports on the ca-pacity of tissue cyst forming of toxoplasma gondii rh strain. in this study, the capac-ity was evaluated by in vivo and in vitro experiments. methods : rh strain was subcutaneously inoculated to ten wistar rats. after one month, their blood, brain, tongue and diaphragm were collected and evaluated by mat, pcr, pathol...

Three-dimensional (3D) cultures are becoming increasingly popular due to their ability to mimic tissuelike structures more effectively than monolayer cultures. In cancer research, the natural tumor characteristics and architecture are more closely mimicked by 3D cell models. Thus, 3D cell cultures are more promising and suitable models, particularly for in vitro drug screening to predict in viv...

this study was conducted to determine the chemical composition, digestibility, degradability and nutritive value of madder, rubia tinctorum l.  and alfalfa medicago sativa. using in vitro and in situ measurements. the mean values for om, cp, ndf, adf and ee were 892.5, 172.0, 306.5, 219.4 and 16.0 g/kg dm for madder hay, and 921.3, 141.4, 482.0, 361.1 and 6.50 g/kg dm for alfalfa hay, respectiv...

background: based on literature, in vitro cholesterol removal of lactic acid bacteria has been accounted for their in vivo cholesterol reduction. but recently, it has been proposed that such in vitro characteristic may not be directly relevant to their in vivo activity. the objective of this study was to find how much in vitro cholesterol reducing potential of lactobacillus plantarum a7 (la7), ...

in this work, we report the effect of biopolymers (starch and gelatin) on in vitro biocompatibility property of nano hydroxyapatite (nhap) composites. cell culture and mtt assays were performed for in vitro biocompatibility. they show that nhap can affect the proliferation of cells and the nhap-starch and nhap-gelatin biocomposites have no negative effect on the cell morphology, viability and p...