Radopholus similis is an important plant parasitic nematode which severely harms many crops. Cathepsin B is present in a wide variety of organisms, and plays an important role in many parasites. Understanding cathepsin B of R. similis would allow us to find new targets and approaches for its control. In this study, we found that Rs-cb-1 mRNA was expressed in esophageal glands, intestines and go...

Transcriptional regulation is an important mechanism underlying gene expression and has played a crucial role in evolution. The number, position and interactions between cis-elements and transcription factors (TFs) determine the expression pattern of a gene. To identify functionally relevant cis-elements in gene promoters, a phylogenetic shadowing approach with a lipase gene (LIP1) was used. As...

The induction of double-strand breaks (DSBs) in plant genomes can lead to increased homologous recombination or site-specific mutagenesis at the repair site. This phenomenon has the potential for use in gene targeting applications in plant cells upon the induction of site-specific genomic DSBs using zinc finger nucleases (ZFNs). Zinc finger nucleases are artificial restriction enzymes, custom-d...

Establishment of a plant-pathogen interaction involves differential gene expression in both organisms. In order to isolate Botrytis cinerea genes whose expression is induced during its interaction with tomato, a comparative analysis of the expression pattern of the fungus in planta with its expression pattern during in vitro culture was performed by differential display of mRNA (DDRT-PCR). Disc...

An endophytic bacterium, Burkholderia cenocepacia 869T2, isolated from vetiver grass, has shown its abilities for both in planta biocontrol and plant growth promotion. Its draft genome sequence was determined to provide insights into those metabolic pathways involved in plant-beneficial activity. This is the first genome report for endophytic B. cenocepacia.

Comparison of the symptoms caused by turnip crinkle virus strain M (TCV-M) and TCV-B infection of a resistant Arabidopsis thaliana line termed Di-17 demonstrates that TCV-B has a greater ability to spread in planta. This ability is due to a single amino acid change in the viral movement protein p8 and inversely correlates with p8 RNA binding affinity.

A interação planta-microrganismos é foco de pesquisas nas últimas décadas. Objetivou-se avaliar a eficiência agronômica do inoculante, contendo Bacillus megaterium e B. subitilis, para soja, via sementes, associado à adubação fosfatada. Conduziu-se quatro ensaios, outubro 2019 março 2020 em Palotina/PR, São Miguel Iguaçu/PR, Cascavel/PR Toledo/PR, com cultivar Piooner® 96y90. Em delineamento bl...

El trabajo de investigación se llevó a cabo en el municipio Papantla del Olarte Veracruz, México con objetivo evaluar dos productos orgánicos, citrato cobre y tierras diatomeas, los cuales fueron aplicados forma foliar 3 diferentes variedades vainilla: Vanilla planifolia Jackson, pompona Schiede, insignis Ame, sistema cultivo malla sombra conformado por camellones establecidos tierra vega fibra...

Os bioativadores podem proporcionar maior disponibilidade de P às plantas. O objetivo deste estudo foi avaliar a resposta da utilização bioativador solo e planta adubação fosfatada nas características rendimento soja. delineamento em blocos casualizados, com quatro repetições, esquema fatorial (5x6), sendo o primeiro fator constituído pela combinação doses superfosfato triplo mais as do (100% S...