The use of non-Saccharomyces yeast for wine making is becoming a common trend in many innovative wineries. The application is normally aimed at increasing aromas, glycerol, reducing acidity, and other improvements. This manuscript focuses on the reproduction of the native microbiota from the vineyard in the inoculum. Thus, native selected yeasts (Hanseniaspora uvarum, Metschnikowia pulcherrima,...

Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only...

In Nigeria, like other developing countries, industries discharge their wastewater without effective treatment due to the high cost of existing treatment technologies. Wastewater containing petroleum hydrocarbon is highly toxic and pose a great danger to the nearby communities. Therefore, there is a need for a more robust and cost effective treatment technology. In this research, a study was ca...

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and dif...

Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicans isolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to bel...

Saccharomyces cerevisiae cannot produce extracellular lipase and utilize low-cost lipid substrates. This study aimed to express extracellular lipase from Yarrowia lipolytica in S. cerevisiae, construct recombinant oily substrate consumer strains, and compare the roles of native and mutant Y. lipolytica extracellular lipases in S. cerevisiae. The LIP2 gene of Y. lipolytica DSM3286 and its mutant...

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

protein-rich hydrolysate from feather waste was obtained using a mixed culture of selected thermophilic actinomycete strains, and was tested for possible application as soil amendment and biological control agent. for this purpose, a 4 months laboratory experiment was carried out using two types of urban soils (sofia, bulgaria): native park soil and anthropogenic soil. the effect of the obtain...