The conventional technique of impression cytology provides a non-invasive method for the evaluation of conjunctival epithelium alterations. Using indirect immunofluorescence procedures two inflammatory markers, class II MHC antigens HLA DR and the receptor to IgE (CD23), were sought in impression cytology specimens obtained from 80 patients. In normal subjects conjunctival epithelial cells did ...

toxoplasmosis is an infection caused by the protozoan parasite toxoplasma gondii (t.gondii) throughout the world. although usually asymptomatic, the infection can cause serious medical problems in immunocompromised individuals and fetuses. toxoplasmosis also causes considerable economic loss because of abortion in livestock. dna vaccination is a promising approach against intracellular parasite...

the ultrastructure of the cells, na+, k+-atpase activity and immunolocalization were examined in the barnchial chamber of libellula lydia (drury, 1773) larvae. na+,k+-atpase activity and localization were performed through biochemical techniques and immunofluorescence light microscopy using a mouse monoclonal antibody iggα5, respectively. the branchial chamber possesses six pair gills lamellae ...

Background: Mesenchymal stem cells (MSCs) are beneficial for cell and gene therapy approaches. These highly potent in producing various cells, including adipocytes, osteoblasts, hematopoietic etc., particularly bone marrow-derived. High proliferation regenerative capacity important phenotypes of MSCs which contribute to the recovery process at damaged sites. Various techniques have been used, i...

Abstract Introduction Atrial fibrillation (AF), the most common cardiac arrhythmia, is associated with increased morbidity and mortality. Inflammatory infiltrates have been observed in atrial tissues from AF patients suggesting that low-grade inflammation contributes to pro-fibrotic, pro-remodeling pro-senescence responses of tissue favoring AF. Although sodium glucose co-transporter2 inhibitor...

Antibodies against chicken and Torpedo agrin were used for immunofluorescent staining in order to assess the spatial distribution and temporal appearance of agrin-like molecules at newly formed synaptic contacts in cultures of embryonic Xenopus nerve and muscle cells. The antibodies stained Xenopus neuromuscular junctions and removed ACh receptor (AChR)-aggregating activity from extracts of Xen...

RATIONALE The standard approach to diagnosis of primary ciliary dyskinesia (PCD) in the United Kingdom consists of assessing ciliary function by high-speed microscopy and ultrastructure by election microscopy, but equipment and expertise is not widely available internationally. The identification of biallelic disease-causing mutations is also diagnostic, but many disease-causing genes are unkno...