objective(s): enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. in this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. materials and methods:  the process consists of the ...

Cellulases are a class of enzymes great industrial interest that present several strategic applications. However, the high cost enzyme production, coupled with instabilities and complexities proteins required for hydrolytic processes, still limits their use in protocols. Therefore, immobilization may be an essential tool to overcome these issues. The work aimed evaluate cellulolytic commercial ...

AaCel9A [β-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. The properties of the immobilized AaCel9A were investigated by determining the optimum pH and optimum temperature for activity, thermal stability, and kinetic parameters. The immobilization process shifted the enzyme’s optimum temperature from 65 °C for the fr...

the abilities of silybum marianum cell culture to biotransform benzaldehyde to benzyl alcohol in three culture systems were compared. callus cultures of silybum marianum were established from seedlings, and healthy suspensions and immobilized cultures grown on the murashige and skoog medium. s. marianum cells were immobilized in both agar beads and fiber cotton matrixes. benzaldehyde was fed to...

Sugar fatty acid esters, especially glucose esters (GEs), have broad applications in food, cosmetic and pharmaceutical industries. In this research, the moieties derived from polyunsaturated acids containing single-cell oils (SCOs) (i.e., those produced Cunninghamella echinulata, Umbelopsis isabellina Nannochloropsis gaditana, as well olive oil an eicosapentaenoic (EPA) concentrate) were conver...