The Genome Sequencer FLX System from Roche and 454 Life SciencesTM is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, metagenomics analysis, and amplicon sequencing. The Genome Sequencer FLX enables long sequence reads separated by kilobase distances of genomic DNA. These Long-Tag Pair...

We used a mixed 454 and Illumina sequencing strategy. Genomic DNA from each House Finch strain was first nebulized and blunt-end ligated with Roche adaptors. Emulsion PCR was performed using the bulk library and these products were pyrosequenced on Roche 454 Gene Sequencer using FLX chemistry, using physical separation of isolates. Genomic DNA from the four newly sequenced poulty strains (Table...

A 3.19-Mbp draft genome of the Gram-positive thermophilic iron-reducing Firmicutes isolate from the Peptococcaceae family, Thermincola ferriacetica Z-0001, was assembled at ~100× coverage from 100-bp paired-end Illumina reads. The draft genome contains 3,274 predicted genes (3,187 protein coding genes) and putative multiheme c-type cytochromes.

Here, we present the draft genome of Planomicrobium glaciei, a member of the phylum Firmicutes, found at the University of California Davis. Paired-end, 300-bp reads were generated on an Illumina MiSeq. The assembly consists of 3,925,122 bp, contained in 109 contigs, with a G+C content of 46.7%.

Induced mutagenesis is a powerful approach to generate variations that can be used toward the elucidation of gene function and create new traits for crop improvement. We have developed pedigreed mutant library through chemical with ethyl methanesulfonate (EMS)-treated seed from sorghum (Sorghum bicolor L. Moench) inbred line BTx623. The displayed wide diversity phenotypes, including potential s...