Objective(s): New generation of allergy vaccines is capable of promoting the development of protective IgG and blocking the functionality of allergen-specific IgE. We incorporated universal and powerful T-cell epitopes from tetanus and diphtheria toxoids (TD epitope) into recombinant Che a 2, the well-known allergic profilin of Chenopodium album, to determine its immun...

The role of interleukin-5 (IL-5) during Toxoplasma gondii infection was investigated by comparing disease progression in IL-5 gene deficient (IL-5-/-) mice and their wild-type (WT) counterparts on a C57BL/6 background. IL-5-/- mice infected orally with T. gondii were less susceptible to infection than WT mice as demonstrated by reduced mortality rates. Consistent with this data, orally infected...

Background Eosinophilic granulomatosis with polyangiitis (EGPA) is a rare form of ANCA-associated vasculitis in which it frequently difficult to achieve glucocorticoid (GC) tapering without relapse. Mepolizumab (MEPO) an approved anti-IL5 agent for EGPA but real-world evidence its long-term efficacy and survival scarce. Objectives To study the efficacy, safety, MEPO daily clinical practice. Met...

Abstract A 49-year-old man without cardiovascular risk factors, recently diagnosed of asthma and rino-sinusal polyposis, entered the Emergency Department for chest pain, shortness breath non-productive cough. The patient was in stable conditions. Laboratory exams showed eosinophilic leucocytosis (WBC 33.000/sqmm, 60% eosinophils) a mild elevation C-reactive protein (CRP 2,2 mg/dL). ECG sinus rh...

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the intestine that encompasses Crohn's disease (CD) and ulcerative colitis. The cause of IBD is unknown, but the evidence suggests that an aberrant immune response toward the commensal bacterial flora is responsible for disease in genetically susceptible individuals. Results from animal models of colitis and human studies in...

In November 2009, in the beautiful city of Venice, Italy, at the first EAACI Pediatric Allergy and Asthma Meeting, I discussed Eosinophilic Esophagitis (EoE) as an emerging disorder. I discussed the diagnosis of the disorder based on histopathologic findings of esophageal eosinophilia of >15 cells per high power field, after 3 months of treatment with a proton pump inhibitors. I discussed sever...

Blockade of inhibitory immune checkpoints (ICPs) improves survival in melanoma patients. Interestingly, the expression of these ICPs in most tumor tissues including melanoma is restricted to only a subset of tumor-infiltrating immune cells (TIICs). Here, we utilize single cell mass cytometry (CyTOF), functional profiling and TCR sequencing of freshly isolated cells to evaluate the phenotypic, f...

Donor shortage remains the single most important factor limiting the success of transplantation medicine. In face of this unrelenting pressure, otherwise prohibitive immunological barriers are increasingly accepted, such as ABO incompatibility of a kidney from an available living donor. Treatment protocols for such situations involve varying combinations of B-cell depletion, removal of isohemag...

Allergic diseases basically are immunologic disorders related to the activation of a distinct cytokine pattern in T cells, including increased secretion of certain allergic in ̄ammatory cytokines, particularly IL-4, IL5, and/or IL-13 (1±3). Whereas the symptoms of immediate and late-type allergic reactions can be ameliorated by various pharmacologic treatments, allergen-speci®c immunotherapy (SI...