Objective: Critical limb ischemia is a major complication of diabetes characterized by insufficient collateral vessel development and proper growth factor signaling unresponsiveness. Although mainly deactivated hypoxia, phosphatases are important players in the deregulation proangiogenetic pathways. Previously, SHP-1 (Scr homology 2-containing phosphatase-1) was found to be associated with down...

RATIONALE While microvascular injury is associated with chronic rejection, the cause of tissue ischemia during alloimmune injury is not yet elucidated. OBJECTIVE We investigated the contribution of T lymphocytes and complement to microvascular injury-associated ischemia during acute rejection of mouse tracheal transplants. METHODS AND RESULTS Using novel techniques to assess microvascular i...

BACKGROUND ZFP580 is a novel C2H2 type zinc-finger transcription factor recently identified by our laboratory. We previously showed that ZFP580 may be involved in cell survival and growth. The aim of this study was to elucidate whether ZFP580 is involved in the cardioprotective effects of intermittent high-altitude (IHA) hypoxia against myocardial ischemia-reperfusion (I/R) injury. METHODS AN...

Global cerebral ischemia/hypoxia, as can occur during human stroke, damages brain neural networks and synaptic functions. The recently demonstrated protein kinase C (PKC) activation-induced synaptogenesis in rat hippocampus suggested the potential of PKC-mediated antiapoptosis and synaptogenesis during conditions of neurodegeneration. Consequently, we examined the effects of chronic bryostatin-...

Background—PR39 is a prolineand arginine-rich peptide implicated in wound healing and myocardial ischemia protection. To determine the potential mechanisms of PR39 in ischemia, we examined the role of PR39 in hypoxia-induced apoptosis in vascular endothelial cells. Methods and Results—Hypoxia results in an increase of apoptosis in bovine aortic endothelial cells (BAECs), as determined by termin...

BACKGROUND PR39 is a proline- and arginine-rich peptide implicated in wound healing and myocardial ischemia protection. To determine the potential mechanisms of PR39 in ischemia, we examined the role of PR39 in hypoxia-induced apoptosis in vascular endothelial cells. METHODS AND RESULTS Hypoxia results in an increase of apoptosis in bovine aortic endothelial cells (BAECs), as determined by te...

PURPOSE To develop a mouse model to study the influence of hypoxia in breast cancer progression and metastasis. METHODS The 4T1 cell line was used to engraft the kidneys of female BALB/c mice. Placing an aneurysm clip on the kidney hilum, hypoxia can be directed to tumor site. Histological evaluation was used to analyze the morphological changes induced by ischemia in kidney cortex, and to ve...

Introduction Peripheral arterial disease (PAD) is a major risk factor for lower-extremity amputation in diabetic patients. Unfortunately, previous clinical studies investigating therapeutic angiogenesis using the vascular endothelial growth (VEGF) have shown disappointing results patients, which evokes necessity novel agents. The apelinergic system (APJ receptor/apelin) highly upregulated under...

Min, Jiang-Yong, Thomas G. Hampton, Ju-Feng Wang, Joseph DeAngelis, and James P. Morgan. Depressed tolerance to fluorocarbon-simulated ischemia in failing myocardium due to impaired [Ca]i modulation. Am J Physiol Heart Circ Physiol 278: H1446–H1456, 2000.—The aim of this study was to investigate the tolerance of failing myocardium from postinfarction rats to simulated ischemia. Myocardial infar...