Gracilariopsis lemaneiformis ( Gp. ) is an economically important agar-producing red alga applicable in the food and cosmetic industries. The genetic knowledge of this species is, however, limited, tools for studying engineering it are lacking. This has limited understanding its developmental genetics hindered development new strains, developing would allow to tackle these problems. Here, trans...

ABSTRACT Endophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of endophytic isolate Acinetobacter lwoffii Bac109 early vitro sugarcane seedlings. The strain showed to solubilize phosphate a solid medium (solubilization index: 3.73). In ad...

Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT), initially restricted to South America, is a global threat for wheat after spreading Asia in 2016 the introduction of contaminated seeds, raising question about transmission pathogen from seeds seedlings, process so far not well understood. We therefore studied relationship between seed infection and disease symptoms on seedlings...

Two mutants of the tylosin-producing Streptomyces fradiae defective in the biosynthesis of the macrolide antibiotic tylosin were isolated from colonies derived from regenerated protoplasts. Both strains were unable to carry out any of at least seven tylosin biosynthetic steps and were sensitive to tylosin. One strain, JS82, was also more sensitive to chloramphenicol (Cm), mitomycin C (Mc), hygr...

The vaccinia virus (VV) E3L protein is essential for virulence and has anti-apoptotic activity. In mice, Z-DNA-binding activity of the N-terminal domain of E3L (Z alpha) is necessary for viral lethality. Here, we report that inhibition of hygromycin-B-induced apoptosis in HeLa cells depends on Z-DNA binding of the E3L Z alpha domain. Z-DNA-binding domains of other proteins are equally effective...

Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms ha...

Stm1p is a Saccharomyces cerevisiae protein that is primarily associated with cytosolic 80S ribosomes and polysomes. Several lines of evidence suggest that Stm1p plays a role in translation under nutrient stress conditions, although its mechanism of action is not yet known. In this study, we show that yeast lacking Stm1p (stm1Delta) are hypersensitive to the translation inhibitor anisomycin, wh...