BACKGROUND RPB5-Mediating protein (RMP) is associated with the RNA polymerase II subunit RPB5. This protein functionally counteracts the transcriptional activation of Hepatitis B Virus X protein (HBx) by competitively binding to the RPB5; however, the effects of RMP on Hepatitis B virus (HBV) transcription and replication remain unknown. OBJECTIVES The purpose of this study was to investigate...

A microfluidic probe (MFP) is a mobile channel-less microfluidic system under which a fluid is injected from an aperture into an open space, hydrodynamically confined by a surrounding fluid, and entirely re-aspirated into a second aperture. Various MFPs have been developed, and have been used for applications ranging from surface patterning of photoresists to local perfusion of organotypic tiss...

Confined granular fluids, placed in a shallow box that is vibrated vertically, can achieve homogeneous stationary states thanks to energy injection mechanisms that take place throughout the system. These states can be stable even at high densities and inelasticities allowing for a detailed analysis of the hydrodynamic modes that govern the dynamics of granular fluids. Analyzing the decay of the...

Diabetes is a disease of abnormal glucose homeostasis characterized by chronic hyperglycemia and a broad array of consequent organ damage. Because normal glucose homeostasis is maintained by a complex interaction between behavior (feeding and physical activity) and metabolic activity that is modulated by inter-organ signaling through secreted factors, disease modeling in vitro is necessarily li...

Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery (HGD) with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcin...

Adoptive transfer of human hematopoietic stem cells (HSCs) into mice lacking T, B and natural killer (NK) cells leads to development of human-blood lineage cells in the recipient mice (humanized mice). Although human B cell reconstitution is robust and T cell reconstitution is reasonable in the recipient mice, reconstitution of NK cells and myeloid cells is generally poor or undetectable. Here,...

Three-dimensional numerical models of potential underground storage and compositional simulation are a way to study the feasibility storing carbon dioxide in existing geological formations. However, results simulations affected by many parameters, we proved that refinement model grid is one them. In this study, impact discretization on CO2 trapping when injected into deep saline aquifer was inv...

The Noyabrsky site is used for the injection of highly mineralized drainage waters formed during development Aikhal kimberlite pipe (Republic Sakha (Yakutia), Russia). water injected into wells since 2013 has a localized intrapermafrost technogenic aquifer. Taking account porosity parameters permafrost reservoirs, as well confirmed useful capacity site, its operation planned to continue until 2...

Hydrodynamic reductions of the hydrodynamic chain associated with dispersionless limit of 2+1 Harry Dym equation are found by the Miura type and reciprocal transformations applied to the Benney hydrodynamic chain.

The new method proposed in this work not only measures the particle size distribution and the average molar mass accurately using the static light scattering (SLS) technique when the Rayleigh-Gans-Debye approximation is valid for dilute poly-disperse homogenous spherical particles in dispersion, but also enables us to have insight into the theoretical analysis of the dimensionless shape paramet...