Suspensions of single cells were prepared from normal adult rat liver either by mincing the tissue and incubating it in various concentrations of sodium tetraphenylborate, ethylenediaminetetraacetate, and 0.1% hyaluronidase plus 0.05% collagenase or by continuous recirculating perfusion of the liver with 0.1% hyaluronidase plus 0.05% collagenase. Only the latter method produced cells in good yi...

With an increase in requests for the correction of aesthetic imperfections with dermal fillers based on hyaluronic acid, cases adverse events and complications have become more frequent. Hyaluronic acid form a filler is one most popular procedures medicine has high safety profile. Despite fact that integral part extracellular matrix, it can also cause complications. The use antidote hyaluronida...

Communication of cells with their extracellular environment is crucial to fulfill their function in physiological and pathophysiological conditions. The literature data provide evidence that such a communication is also important in case of astrocytes. Mechanisms that contribute to the interaction between astrocytes and extracellular matrix (ECM) proteins are still poorly understood. Hyaluronan...

It has been reported previously that the dermal spread of India ink with hyaluronidase is markedly influenced by the hormones of the adrenal cortex.'6' Removal of the adrenals resulted in an enhancement of the spreading reaction, while the administration of a potent extract of the adrenal cortex either in the adrenalectomized or normal animal inhibited spreading. However, desoxycorticosterone a...

BACKGROUND Failed back surgery syndrome (FBSS) has been reported to account for up to 40% of patients with chronic low back pain. Epidural fibrosis may be responsible for up to 30% of all cases of FBSS. Perineural fibrosis can interfere with cerebrospinal fluid-mediated nutrition, rendering the nerve root hyperesthetic and hypersensitive to compression. Traditionally, steroid injection has been...

Hyaluronidase release was used as an index of acrosomal membrane damage during cold shock of epididymal boar sperm and ejaculated sperm from intact and vesiculectomized boars. Sperm were also incubated with seminal plasma from intact and vasectomized boars to examine the contributions of male accessory gland secretions. Acrosomal membranes of epididymal sperm were more resistant to cold shock t...