نتایج جستجو برای: hyaluronan binding assay

تعداد نتایج: 616266  

Journal: :Nagoya journal of medical science 2005
Yuko Naito Noriko Suzuki Pengyu Huang Hitoki Hasegawa Yasuyoshi Sohara Takashi Iwamoto Michinari Hamaguchi

Malignant transformation of cells is frequently associated with an augmented production of hyaluronan and the subsequent formation of a hyaluronan-matrix. In v-Src-transformed cells, hyaluronan directly activate cell motility in a tumor-specific manner. Despite its importance, the mechanism by which v-Src activates hyaluronan production remains unclear. Here we report that multiple signaling pa...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2010
J A Sloane C Batt Y Ma Z M Harris B Trapp T Vartanian

Failure of remyelination is largely responsible for sustained neurologic symptoms in multiple sclerosis (MS). MS lesions contain hyaluronan deposits that inhibit oligodendrocyte precursor cell (OPC) maturation. However, the mechanism behind this inhibition is unclear. We report here that Toll-like receptor 2 (TLR2) is expressed by oligodendrocytes and is up-regulated in MS lesions. Pathogen-der...

Journal: :The Journal of biological chemistry 2015
Geneviève Bart Nuria Ortega Vico Antti Hassinen Francois M Pujol Ashik Jawahar Deen Aino Ruusala Raija H Tammi Anthony Squire Paraskevi Heldin Sakari Kellokumpu Markku I Tammi

In vertebrates, hyaluronan is produced in the plasma membrane from cytosolic UDP-sugar substrates by hyaluronan synthase 1-3 (HAS1-3) isoenzymes that transfer N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcUA) in alternative positions in the growing polysaccharide chain during its simultaneous extrusion into the extracellular space. It has been shown that HAS2 immunoprecipitates contain f...

Journal: :Investigative ophthalmology & visual science 2008
Akiko Ohno-Jinno Zenzo Isogai Masahiko Yoneda Kenji Kasai Osamu Miyaishi Yoko Inoue Takuya Kataoka Jing-Song Zhao Huili Li Masayuki Takeyama Douglas R Keene Lynn Y Sakai Koji Kimata Masayoshi Iwaki Masahiro Zako

PURPOSE In this study, biochemistry, molecular biology, immunohistochemistry, and electron microscopy techniques were used to examine whether versican, which is known to bind fibrillin-1, interacts with fibrillin-1 in the ciliary body and vitreous, and whether the versican in this complex binds to hyaluronan. METHODS The new polyclonal antibodies against the amino and carboxyl termini of vers...

2016
Dong-Ki Kim Hosoon Choi Hidetaka Nishida Joo Youn Oh Carl Gregory Ryang Hwa Lee Ji Min Yu Jun Watanabe Su Yeon An Thomas J. Bartosh Darwin J. Prockop Irina Kerkis

TNF-α stimulated gene/protein 6 (TNFAIP6/TSG-6) is a multifunctional protein that has a number of potential therapeutic applications. Experiments and clinical trials with TSG-6, however, have been limited by the technical difficulties of producing the recombinant protein. We prepared stable clones of CHO cells that expressed recombinant human TSG-6 (rhTSG-6) as a secreted glycoprotein. Paradoxi...

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