OBJECTIVE To study the clonality of cytogenetically normal progenitors detected in the peripheral blood (PB) of acute myeloid leukemia (AML) patients. METHODS Five female patients with cytogenetically abnormal, newly-diagnosed AML who were heterozygous for informative alleles of the androgen receptor (AR) gene were studied using the human androgen receptor allele (HUMARA) assay. RESULTS PB ...

A patient presenting with severe malaria, hyperparasitaemia, received 7-day artesunate monotherapy. recrudescence was detected and attributed to monotherapy a polyclonal infection without Kelch 13 gene mutation. second treatment artesunate, then quinine, followed by artemether-lumefantrine, successful.

AIMS AND BACKGROUND NYD-SP8 is a recently identified protein, the biological characteristics of which are still unclear. The aim of this study was to prepare an anti-NYD-SP8 rabbit polyclonal antibody and investigate the expression of NYD-SP8 in human nasopharyngeal carcinoma cell lines and nasopharyngeal carcinoma tissues. METHODS The anti-NYD-SP8 rabbit polyclonal antibody was prepared and ...

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

BACKGROUND We investigated the utility of bioluminescence imaging (BLI) using firefly luciferase in monoclonal and polyclonal populations of leukemia cells in vitro and in vivo. METHODS Monoclonal and polyclonal human lymphoid and myeloid leukemia cell lines transduced with firefly luciferase were used for BLI. RESULTS Kinetics and dynamics of bioluminescence signal were cell line dependent...

Coagulase-negative staphylococcus (CNS) is the most frequent cause of nosocomial bacteremia and prosthetic valve endocarditis. CNS bacteremia can be polyclonal. No data exist on the clonality of CNS causing endocarditis. We present a case of CNS aortic homograft endocarditis in which at least five different genotypes of CNS were identified in initial blood-culture isolates by genomic macrorestr...

The extensive application of the hybridoma technology for tissue phenotyping has demonstrated in the last decade that a number of heterodimeric molecules consisting of noncovalently associated α and β subunits (Hynes, 1992), named integrins, play a major role in cell-to-cell and cellto-interstitium interactions as well as in cell trafficking (Hemler, 1990; Albelda and Buck, 1990). The structura...