Background: The effect of electromagnetic field (EMF) as an environmental factor on different organs including female reproductive system is of critical concern. In view of the fact that embryo fetuses and young, growing animals are more susceptible to any xenobiotic during gestational and lactation stages of gonadal development may it lead to permanent damage to the gonads. The early phases of...

objective: cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. the aim of this study was to investigate the effects of fibroblast growth factor (fgf) and epidermal growth factor (egf) on in vitro pre...

Background The best time of final oocyte maturation triggering in assisted reproduction technology protocols is unknown. This time always estimated by combined follicular size and blood progesterone level. Objective The aim of this study was evaluation of the effect of delaying oocyte maturation triggering by 24 hr on the number of mature oocytes (MII) and other in vitro fertilization cycle c...

background: the purpose of this study was to compare the three different strategies, intravenous (iv) hydroxylethyl starch (hes), iv human albumin (ha), and oral cabergoline (cb) in the prevention of ovarian hyperstimulation syndrome (ohss). materials and methods: in this prospective randomized clinical trial, 91 women at high risk of developing ohss were allocated into the three groups, group ...

BACKGROUND Controlling nuclear maturation during oocyte culture might improve nuclear-cytoplasmic maturation synchrony. In the present study, the quality of mouse and human cumulus-enclosed oocytes (CEOs) was examined after a two-step culture consisting of a three-dimensional prematuration culture (3D-PMC), followed by in vitro maturation (IVM). METHODS Mouse and human CEOs were embedded in a...

In vitro maturation (IVM) of oocytes is a promising technology for both the treatment of human infertility and in animal production as a means of improving genetic gain. However, IVM derived oocytes remain inferior to those matured in vivo with reduced developmental potential. The environment in which an oocyte matures in vitro is vastly different to in vivo where maturation takes place within ...

BACKGROUND Recently we reported that an oocyte activation ability in human and mouse sperm is associated with head flatness or the presence of perinuclear theca (PT) substance, MN13, which is an oocyte activation-related protein localized on the post-acrosomal sheath (PAS). As such, we hypothesize that the appearance of oocyte activation ability is stage-specifically regulated and depends on th...

BACKGROUND Live birth per cycle and live birth per embryo transfer are commonly used outcome measures for IVF treatment. In contrast, the assessment of the reproductive efficiency of human oocytes fertilized in vitro is seldom performed on an egg-to-egg basis. This approach may however gain importance owing to the increasingly widespread use of oocyte cryopreservation, as the technique is becom...

Oocyte maturation is associated with suppression of transcriptional activity. Consequently, gene expression during oocyte maturation, fertilization and early embryo development, until zygotic genome activation (ZGA) is primarily regulated by translational activation of maternally derived mRNAs. Embryonic poly(A)-binding protein (EPAB) is the predominant poly(A)-binding protein in Xenopus, mouse...