Endothelial progenitor cells (EPCs) are both reduced and dysfunctional in hypertension that correlates inversely with its mortality, but the mechanisms are poorly understood. Endothelial nitric oxide synthase (eNOS) critically regulates EPC mobilization and function but is uncoupled in salt-sensitive hypertension because of the reduced cofactor tetrahydrobiopterin (BH4). We tested the hypothesi...

The fission yeast Schizosaccharomyces pombe is an attractive model organism for studying eukaryotic biology due to the ease of genetic and molecular manipulations (3). In recent years, several selectable markers and promoters have been developed to enhance our ability to manipulate gene expression in this yeast. Recently, Invitrogen (Carlsbad, CA, USA) has developed the SpECTRA S. pombe Expres...

Helper-dependent adenoviral vectors (HDAds) possess long homology arms that mediate high-efficiency gene editing. These long homology arms may permit simultaneous introduction of multiple modifications into a large genomic region or may permit a single HDAd to correct many different individual mutations spread widely across a gene. We investigated this important potential using an HDAd bearing ...

The development of selectable markers for transformation has been a major factor in the successful genetic manipulation of plants. A new selectable marker system has been developed based on bacterial gentamicin-3-N-acetyltransferases [AAC(3)]. These enzymes inactivate aminoglycoside antibiotics by acetylation. Two examples of AAC(3) enzymes have been manipulated to be expressed in plants. Chime...

The Janus cassette permits marker-free allelic replacement or knockout in streptomycin-resistant Streptococcus pneumoniae (pneumococcus) through sequential positive and negative selection. Spontaneous revertants of Janus can lead to high level of false-positives during negative selection, which necessitate a time-consuming post-selection screening process. We hypothesized that an additional cou...

The green microalgae Chlorella vulgaris is a source of valuable nutrients, whose bioaccessibility limited by the structurally complex cell wall. Enzymatic degradation wall represents remarkable alternative to mechanical treatments due its mildness and specificity. This work aimed define an optimal combination enzymes increase lipid protein bioaccessibilities C. cells while preserving oxidative ...