The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alphaC, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-S...

The addition of gibberellic acid to isolated aleurone layers of barley (Hordeum vulgare L.) causes the production and secretion of four alpha-amylases. Two of these are stable at pH 3.7 and are not inactivated by ethylenediaminetetraacetate. The other two represent the classical barley alpha-amylases; i.e., they are inactivated at pH 3.7 and by reagents which from complexes with divalent metal ...

Adenine phosphoribosyltransferase activity was measured in extracts of soybean (Glycine max var. Acme) callus and of senescing barley leaves (Hordeum distichon c.v. Prior). The enzyme from soybean callus had Michaelis constants for adenine and 5-phosphoribosyl pyrophosphate of 1.5 and 7.5 mum respectively and was inhibited by AMP and stimulated by ATP. The presence of kinetin was found to consi...

The 9-kDa thylakoid polypeptide which in vivo carries the iron-sulfur centers A and B of photosystem I was isolated from barley (Hordeum vulgare L.) and the complete amino acid sequence determined. The polypeptide shows a very high degree of homology with the corresponding polypeptides in other plant species. The polypeptide is not post-translationally processed except for the removal of the N-...