In this paper, we derive a method to refine a Bayes network diagnostic model by exploiting constraints implied by expert decisions on test ordering. At each step, the expert executes an evidence gathering test, which suggests the test’s relative diagnostic value. We demonstrate that consistency with an expert’s test selection leads to non-convex constraints on the model parameters. We incorpora...

Complete List of Authors: Camassa, Roberto; The University of North Carolina at Chapel Hill, Department of Mathematics Lin, Zhi; Zhejiang University, Department of Mathematics McLaughlin, Richard; University of North Carolina at Chapel Hill, Department of Mathematics Mertens, Keith; University of North Carolina at Chapel Hill, Department of Mathematics Tzou, Chung-Nan; The University of North C...

1 The MathWorks, Inc., Simulink Development, 3 Apple Hill Dr., Natick, MA 01760-2098, U.S.A. 2 The MathWorks, Inc., Application Engineering, 39555 Orchard Hill Place, Novi, MI 48375-5374, U.S.A. 3 The MathWorks, Inc., xPC Target Development, 3 Apple Hill Dr., Natick, MA 01760-2098, U.S.A. 4 The MathWorks, Inc., Technical Marketing, 39555 Orchard Hill Place, Novi, MI 48375-5374, U.S.A. 5 The Mat...

Enzyme kinetics for systems biology should ideally yield information about the enzyme's activity under in vivo conditions, including such reaction features as substrate cooperativity, reversibility and allostery, and be applicable to enzymatic reactions with multiple substrates. A large body of enzyme-kinetic data in the literature is based on the uni-substrate Michaelis-Menten equation, which ...

The reaction catalyzed by purified rat liver cytosol tyrosine aminotransferase (L-tyrosine + 2 oxoglutarate = p-hydroxyphenylpyruvate + L-glutamate, EC 2.6.1.5) has been analyzed with regard to product inhibition and combination with each of its rate-limiting substrates by the Hill equation. Glutamate is a linear competitive inhibitor with respect to tyrosine, a linear noncompetitive inhibitor ...

Activation and desensitization kinetics of the rat P2X1 receptor at nanomolar ATP concentrations were studied in Xenopus oocytes using two-electrode voltage-clamp recording. The solution exchange system used allowed complete and reproducible solution exchange in <0.5 s. Sustained exposure to 1-100 nM ATP led to a profound desensitization of P2X1 receptors. At steady-state, desensitization could...

SimnlIrv(11'. 1lii order to avoid the l)roblelll of ani uncouplinig effect in stimiiulatioln of potential Hill reaction activity durinig extractioln with heptane, Hill reaction activity was measured with uncouple(d spinach chloroplasts. TI'he reactions were also runi ulnder aerobic coniditionis. Under these conditions a correctioni for a 3(p-chloroph'enyl) -1. 1-dimethylurea insensitive, photos...