نتایج جستجو برای: high resolution melt analysis
تعداد نتایج: 4541240 فیلتر نتایج به سال:
dna methylation plays an important role in carcinogenesis through epigenetic silencing of tumor suppressor genes. aberrant methylation usually results from changes in the activity of dna methyltransferases (dnmts). some studies show that the overexpression of the dnmts may lead to aberrant methylation of tumor suppressor genes. also the overexpression of dnmts may be related to methylation stat...
Foodborne pathogens pose risks to populations all over the world. Pathogens can be used as bioterrorism agents, causing an outbreak that affects many individuals through consumption of a commonly affected food or beverage. A PCR assay identify their unique melting points using high-resolution melt assay. Assays detect bacteria individually from mixture species-specific primers. An was developed...
Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, novel real-time PCR-high-resolution melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling were developed. For the detection of mutations ...
BACKGROUND Real-Time PCR-High Resolution Melting (qPCR-HRM) analysis has been recently described for rapid drug susceptibility testing (DST) of Mycobacterium leprae. The purpose of the current study was to further evaluate the validity, reliability, and accuracy of this assay for M. leprae DST in clinical specimens. METHODOLOGY/PRINCIPAL FINDINGS The specificity and sensitivity for determinin...
When short data records are available, spectral analysis is basically an undetermined linear inverse problem. One usually considers the theoretical setting of regularization to solve such ill-posed problems. In this paper, we rst show that "nonparametric" and "high resolution" are not incompatible in the eld of spectral analysis. To this end, we introduce non quadratic convex penalization funct...
This article is concerned with the use of unsupervised methods to process very high resolution satellite images with minimal or little human intervention. In a context where more and more complex and very high resolution satellite images are available, it has become increasingly difficult to propose learning sets for supervised algorithms to process such data and even more complicated to proces...
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