The power of traditional high-performance liquid chromatography with UV detection (HPLC-UV, DAD) can be readily extended by simply coupling a mass spectrometer (MS) to an existing system. In the early years of liquid chromatographic mass spectrometry (LC-MS), this coupling was considered exotic and complex. After more than 15 years of refinement, LC-MS systems are robust and easy to use, and pr...

This work describes the application of ultrasound-assisted matrix solid-phase dispersion as an extraction and sample preparation approach for aflatoxins (B1, B2, G1 and G2) and subsequent determination of them by high-performance liquid chromatography-fluorescence detection. A Box-Behnken design in combination with response surface methodology was used to determine the affecting parameters on t...

In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...

A simple, rapid and sensitive method termed as magnetic solid phase extraction (MSPE) combined with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) has been proposed for the determination of trace amounts of chlorpromazine (CPZ) in water, urine and plasma samples. The separation and determination was performed on a C18 column under the optimal chromatographic conditions. S...

Poly-L-glutamic acids can exist in random coil, ibility because of its poor pH stability and organic helix, or sheet depending on the environmental conditions. Therefore, they have been used as molecular models for a better understanding of macromolecular stereochemistry [1]. Polyglutamylation is also the major posttranslational modiWcation of neuronal tubulin [2,3], and foliates are polyglutam...

INTRODUCTION Peak and trough serum concentrations of vancomycin were determined in term newborn infants with confirmed or suspected Staphylococcus sp sepsis by high performance liquid chromatography and flourescence polarization immunoassay. OBJECTIVE To statistically compare the results of the high performance liquid chromatography and flourescence polarization immunoassay techniques for mea...

the total vitamin c content in human plasma is widely accepted as an indicator of the tissue status of vitamin c. a liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. a c18 reversed-phase column and cetrimide as an ion-pairing agent was employed. ascorbic acid (aa) was measured after reducing l-dehydroascorbic acid to l-asc...