Polyclonal antibodies were raised against Heterodera glycines eggshells to determine the feasibility of developing an immunoassay for H. glycines eggs. An indirect enzyme-linked immunosorbent assay (ELISA) was developed from anfisera collected 10 weeks after the initial injection. From serial dilutions of sonicated eggshells or whole eggs, a sensitivity of detection to 5 ng/ml sonicated eggshel...

Three populations of the corn cyst nematode Heterodera zeae, one found in the rhizosphere of a fig tree and two infecting corn, were studied using the morphology and morphometry of cysts and second-stage juveniles, and compared with other populations. The intrapopulation and intraspecific variability are discussed. A simple and improved technique to prepare vulval cones for SEM is described. Th...

Nematodes are roundworms that constitute the phylum Nematoda. Only a small fraction of nematode genera contains plant-parasitic or animal-parasitic species, while the majority of nematodes are free-living [1]. Heterodera glycines, the soybean cyst nematode, is a plant-parasitic nematode causing major damage to soybean production worldwide. Annual United States yield loss estimates due to H. gly...

Progeny from single females of four known races of Heterodera glycines Ichinohe were used to establish relatively uniform populations. Single females from these populations were mated with males of other races in all possible combinations to study compatibility and inheritance patterns. When race 1 or 3 was crossed with either race 2 or 4, there was a significant reduction in number of females ...