By D. L. RUCKNAGEL, E. B. PAGE AND W. N. JENSEN S TUDIES OF HEMOGLOBIN by the various technics of electrophoresis aud alkali denaturatiomi have resulted imi a strikimig development in the knowledge of abnormal hemoglobins amid in the hereditary hemolytic amieniias, some of them never previously recognized. During the course of the examination of the electrophoretic properties of the hemoglobimi...

3. Taher A, Isma'eel H, Cappellini MD. Thalassemia intermedia: revisited. Blood Cells Mol Dis 2006;37:12-20. 4. Huehns ER, Dance N, Beaven GH, Heclht F, Motulsky AG. Human embryonic hemoglobins. Cold Spring Harb Symp Quant Biol 1964;29:327-31. 5. Villegas A, Ropero P, González FA, Anguita E, Espinós D. The thalassemia syndromes: molecular characterization in the Spanish population. Hemoglobin 2...

Incubation of human serum with D-[6-3H]glucose resulted in the gradual accumulation of radioactivity in acid-precipitable material. Upon chromatography on Sephadex G-200, radioactivity was found associated with each of the major molecular weight classes of serum protein. Purified human serum albumin was also glucosylated in vitro upon exposure to D-[6-3H]glucose in phosphate-buffered saline. Th...

background: chronic kidney disease is a progressive and irreversible loss of renal function. anemia is one of the main complications of end-stage renal disease (esrd) which is linked closely with other complications of the disease. the primary therapy for anemia in these patients is erythropoietin (epo). the goal of this study was to find the effect of vitamin d administration in addition to th...

BACKGROUND Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 12 current assay methods. METHODS Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on one enzymatic, nine ion-exchange HPLC and two Capillary Electrophoresis methods. Trinity ultra(2) boronate affinity HPLC was the...

We compare and discuss three electrophoretic methods for identifying hemoglobins S, A, C, F, and D or G. Electrophoresis on citrate agar gel was more sensitive than electrophoresis on cellulose acetate for detecting hemoglobins S and F, a fundamental consideration in designing cord-blood screening programs for detecting hemoglobin S carriers. Electrophoresis on starch gel is evidently an accept...

We have recently identified and characterized NADPH-dependent microsomal heme oxygenase as the major enzymatic mechanism for the conversion of hemoglobin-heme to bilirubin-IXalpha in vivo. Enzyme activity is highest in tissues normally involved in red cell breakdown, that is, spleen, liver, and bone marrow, but it usually is negligible in the kidney. However, renal heme oxygenase activity may b...