Second-harmonic generation (SHG) is the most specific label-free indicator of collagen accumulation in widespread pathologies such as fibrosis, and SHG-based measurements hold important potential for biomedical analyses. However, efficient collagen SHG scoring in histological slides is hampered by the limited depth-of-field of usual nonlinear microscopes relying on focused Gaussian beam excitat...

In this perspective, we discuss how the nonlinear optical technique of second-harmonic generation (SHG) microscopy has been used to greatly enhance our understanding of the tumor microenvironment (TME) of breast and ovarian cancer. Striking changes in collagen architecture are associated with these epithelial cancers, and SHG can image these changes with great sensitivity and specificity with s...

Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of these two entities are of high interest. An accurate diagnosis at biopsy is im...

Snyder, Gregory R. M.S., Purdue University, May 2014. Applications of Calculated Second Harmonic Generation Tensors on Monomolecular and Bimolecular Systems. Major Professor: Garth J. Simpson. The field of nonlinear optics (NLO) has proven itself as a relatively recent and powerful area of science in the areas of microscopy and high-sensitivity quantification measurements. In particular, symmet...

Characteristics of high-power, narrow-linewidth, continuous-wave (cw) green radiation obtained by simple single-pass second-harmonic-generation (SHG) of a cw ytterbium fiber laser at 1064 nm in the nonlinear crystals of PPKTP and MgO:sPPLT are studied and compared. Temperature tuning and SHG power scaling up to nearly 10 W for input fundamental power levels up to 30 W are performed. Various con...

Polarization-resolved second harmonic generation (P-SHG) microscopy is an efficient imaging modality for in situ observation of biopolymers structure in tissues, providing information about their mean in-plane orientation and their molecular structure and 3D distribution. Nevertheless, P-SHG signal build-up in a strongly focused regime is not throroughly understood yet, preventing reliable and ...

A new nonlinear optical (NLO) material, RbNa5Ca5(CO3)8, has been synthesized by the hydrothermal method. The crystal structure is established by single-crystal X-ray diffraction. RbNa5Ca5(CO3)8 crystallizes in the hexagonal crystal system with space group P63mc (No. 186). The structure of RbNa5Ca5(CO3)8 can be described as the adjacent infinite [CaCO3]∞ layers lying in the a-b plane bridged thr...

Introduction The aging process causes changes in the sleep-wake cycle and cognition, especially executive functions. Interventions are required to minimize the impact of the losses caused by the aging process. Objective To evaluate the effects of a cognitive training program and psychoeducation on sleep hygiene techniques for executive functions and sleep quality in healthy elderly. Methods...