نتایج جستجو برای: hek 293t
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چکیده فارسی سلولهای پرتوان القایی همانند سلولهای بنیادی توانایی تقسیم نامحدود و تمایز به انواع ردههای سلولی را دارند. این سلولها از بازبرنامه ریزی ژنومی سلولهای تمایز یافته ای بدست می آیند که ژنهای پرتوانی در آنها مجدداً روشن شده است. در این پایان نامه سلولهای ips ای تولید شده است که با سیستم القاء شونده با داکسی سایکلین بیان ژنهای پرتوان خارجی در آن قابل کنترل است. از سلولهای ips تولید شده برا...
To study the relationship between arsenic resistance of 293T cells and overexpression of ARG1, the ARG1 gene in a recombinant plasmid was transfected into 293T cells via liposomes, and then ARG1 overexpression was examined by real-time PCR and immunocytochemistry. The survival rate, arsenic accumulation and arsenic efflux, GSH level, and GST activity of 293T cells overexpressing ARG1 were assay...
Background: Pilot and large-scale production of recombinant proteins requires the presence of stable clones capable of producing large quantities of recombinant proteins. Not only the process of selecting stable clones is time consuming, but also the continuous culturing of clones in large-scale production may cause loss of incoming plasmid and recombinant genes. Thus, considering the advanceme...
Background: The studies show that the Diclofenac and Ibuprofen drugs influence cancer cells proliferation. The main aim of this study was to investigate the effects of Diclofenac and Ibuprofen on the viability of HEK cells in cell culture. Materials and Methods: In this laboratory experimental study, HEK cells were randomly divided into control group and groups exposed doses to 0.001,0/01,0/...
GABAA receptors are responsible for fast inhibitory neurotransmission in the mammalian brain and are the targets for many clinical drugs that act as antiepileptics, anxiolytics, and sedatives. The pharmacological characteristics of the receptor are largely determined by its subunit composition. Compared with all other subtypes, the 6 subtype confers unique pharmacological properties. In particu...
Background & aim: Nanotechnology is a modern research field with broad applications in cancer management. Among the various metal nanoparticles, silver nanoparticles (AgNPs) have been used in cancer therapy due to their promising anti-tumor properties. Despite the great advantages of AgNPs, their effects on normal cells have become a challenge. Besides, their anti-cancer effects have not previo...
Polymerase of influenza virus is made up three subunits PB1, PB2, and PA, which are involved in viral genome transcription replication. Purification sufficient amounts polymerase essential to understand the catalytic function polymerase. In this study, we generated a expression system human embryonic kidney 293T cells (293T cells). The cDNAs for RNA segments 1, 2, 3, encode PA proteins, respect...
Resistance to etoposide has been associated with the overexpression of P-glycoprotein and MRP1 in human tumor cells. However, the role of BCRP in resistance to etoposide has not been clearly established, especially the significance of arginine 482 mutations in drug transport (cellular uptake and efflux). Different levels of resistance to etoposide...
40 Genetic alterations in the carboxypeptidase A1 gene (CPA1) are associated with 41 early-onset chronic pancreatitis (CP). Besides CPA1, there are two other human pancreatic 42 carboxypeptidases: CPA2 and CPB1. Here we examined whether CPA2 and CPB1 alterations 43 are associated with CP in Japan and Germany. All exons and flanking introns of CPA2 and 44 CPB1 were sequenced in 477 Japanese pati...
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