Having opposing enzymatic activities, histone acetylases (HATs) and deacetylases affect chromatin and regulate transcription. The activities of the two enzymes are thought to be balanced in the cell by an unknown mechanism that may involve their direct interaction. Using fluorescence resonance energy transfer analysis, we demonstrated that the acetylase PCAF and histone deacetylase 1 (HDAC1) ar...

Digital aerial triangulation is increasingly being carried out in photogrammetric production environments as greater efficiency is being attained through increased automation. In this paper, a customised and modified approach to digital aerial triangulation using the Helava Automated Triangulation System (HATS) is introduced. HATS is used as the basic triangulation measurement tool for the aeri...

The purpose of this study was to elucidate the mechanisms by which histone acetylation participates in transcriptional regulation of hsp70 (heat-shock protein 70) genes SSA3 and SSA4 in yeast. Our results indicated that histone acetylation was required for the transcriptional activation of SSA3 and SSA4. The HATs (histone acetyltransferases) Gcn5 (general control non-derepressible 5) and Elp3 (...

Metabolic enzymes rarely regulate informational processes like gene expression. Yeast acetyl-CoA synthetases (Acs1p and 2p) are exceptional, as they are important not only for carbon metabolism but also are shown here to supply the acetyl-CoA for histone acetylation by histone acetyltransferases (HATs). acs2-Ts mutants exhibit global histone deacetylation, transcriptional defects, and synthetic...

The molecular machinery that governs circadian rhythmicity comprises proteins whose interplay generates time-specific transcription of clock genes. The role of chromatin remodeling in a physiological setting such as the circadian clock is yet unclear. We show that the protein CLOCK, a central component of the circadian pacemaker, has histone acetyltransferase (HAT) activity. CLOCK shares homolo...

We describe the development of a speech recognition system for conversational telephone speech (CTS) that incorporates acoustic features estimated by multilayer perceptrons (MLP). The acoustic features are based on frame-level phone posterior probabilities, obtained by merging two different MLP estimators, one based on PLP-Tandem features, the other based on hidden activation TRAPs (HATs) featu...

Transcription factors bind to regulatory elements in DNA and activate or suppress gene transcription. DNA is tightly wound around histone proteins, however, and before transcriptional regulators can bind, histones must loosen their grip. This is achieved by acetylation,whichismediatedbyhistoneacetyltransferases (HATs) and reversed by histone deacetylases (HDACs). Both HATs and HDACs have roles ...

The Household Activity-Travel Simulator is a survey technique which is designed to investigate households' everyday decisions about their use of transport and land-use facilities. The interview procedures and survey equipment are described and examples given of different applications of the technique. These are wide ranging and include educational, research, and policy-oriented studies. Introdu...

The Abstract Behavioral Specification (ABS) language is a formal, executable, object-oriented, concurrent modeling language intended for behavioral modeling of complex software systems that exhibit a high degree of variation, such as software product lines. We give an overview of the architectural aspects of ABS: a feature-driven development workflow, a formal notion of deployment components fo...

This paper investigates the optimal signal detection problem with a particular interest in large-scale multiple-input multiple-output (MIMO) systems. The is NP-hard and can be solved optimally by searching shortest path on decision tree. Unfortunately, existing search algorithms often involve prohibitively high complexities, which indicates that they are infeasible MIMO To address this issue, w...